Data & Images
|Product Name||Anti-LRP1/Lrp 1 Cluster Ii Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Prolow-density lipoprotein receptor-related protein 1(LRP1) detection. Tested with WB in Human.|
|Cite This Product||Anti-LRP1/Lrp 1 Cluster Ii Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9143)|
|Replacement Item||This antibody may replace the following items: sc-16166|sc-25469|sc-19616|sc-16168|sc-57353|sc-57352|sc-57351 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human LRP1 recombinant protein (Position: A4351-A4544). Human LRP1 shares 86% amino acid (aa) sequence identity with mouse LRP1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Prolow-density lipoprotein receptor-related protein 1|
|Molecular Weight||504606 MW|
|Protein Function||Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.|
|Tissue Specificity||Most abundant in liver, brain and lung.|
|Sequence Similarities||Belongs to the LDLR family.|
|Subcellular Localization||Low-density lipoprotein receptor-related protein 1 85 kDa subunit: Cell membrane; Single-pass type I membrane protein. Membrane, coated pit.|
|Alternative Names||Prolow-density lipoprotein receptor-related protein 1;LRP-1;Alpha-2-macroglobulin receptor;A2MR;Apolipoprotein E receptor;APOER;CD91;Low-density lipoprotein receptor-related protein 1 85 kDa subunit;LRP-85;Low-density lipoprotein receptor-related protein 1 515 kDa subunit;LRP-515;Low-density lipoprotein receptor-related protein 1 intracellular domain;LRPICD;LRP1;A2MR, APR;|
|Research Areas|||neuroscience|neurology process|neurodegenerative disease|alzheimer's disease| cardiovascular|blood|serum proteins|lipids / lipoproteins|lipid metabolism|cholesterol metabolism| cell biology|apoptosis|phagocytosis| signal transduction|atherosclerosis|lipoprotein metabolism| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|metabolism processes|types of disease|obesity|cancer|heart disease| cancer|cell death||
Background for Prolow-density lipoprotein receptor-related protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-LRP1/Lrp 1 Cluster Ii Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-LRP1/Lrp 1 Cluster Ii Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti LRP1 (PB9143) at 0.5ug/ml
WB: Recombinant Human LRP1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,