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SKU:PB9143
Clonality:Polyclonal
Application:WB
Price: $240.00
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Overview

Product Name Anti-LRP1 Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Prolow-density lipoprotein receptor-related protein 1(LRP1) detection. Tested with WB in Human.
Cite This Product Anti-LRP1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9143)
Replacement Item This antibody may replace the following items: sc-16166|sc-25469|sc-19616|sc-16168|sc-57353|sc-57352|sc-57351 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human LRP1 recombinant protein (Position: A4351-A4544). Human LRP1 shares 86% amino acid (aa) sequence identity with mouse LRP1.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name LRP1
Protein Name Prolow-density lipoprotein receptor-related protein 1
Molecular Weight 504606 MW
Protein Function Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Tissue Specificity Most abundant in liver, brain and lung.
Sequence Similarities Belongs to the LDLR family.
Subcellular Localization Low-density lipoprotein receptor-related protein 1 85 kDa subunit: Cell membrane; Single-pass type I membrane protein. Membrane, coated pit.
Uniprot ID Q07954
Alternative Names Prolow-density lipoprotein receptor-related protein 1;LRP-1;Alpha-2-macroglobulin receptor;A2MR;Apolipoprotein E receptor;APOER;CD91;Low-density lipoprotein receptor-related protein 1 85 kDa subunit;LRP-85;Low-density lipoprotein receptor-related protein 1 515 kDa subunit;LRP-515;Low-density lipoprotein receptor-related protein 1 intracellular domain;LRPICD;LRP1;A2MR, APR;
Research Areas |neuroscience|neurology process|neurodegenerative disease|alzheimer's disease| cardiovascular|blood|serum proteins|lipids / lipoproteins|lipid metabolism|cholesterol metabolism| cell biology|apoptosis|phagocytosis| signal transduction|atherosclerosis|lipoprotein metabolism| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|metabolism processes|types of disease|obesity|cancer|heart disease| cancer|cell death|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Prolow-density lipoprotein receptor-related protein 1

LRP1, also known as Low density lipoprotein receptor-related protein 1 or CD91, is a protein forming a receptor found in the plasma membrane of cells involved in receptor-mediated endocytosis. It is mapped to 12q13.3. In humans, the LRP1 protein is encoded by the LRP1 gene. It is mapped to 12q13.3. LRP1 is involved in several cellular processes, including intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. LRP1 showed strong calcium binding. It mediates the endocytosis and degradation of secreted amyloid precursor protein, suggesting that a single metabolic pathway links 2 molecules implicated in the pathophysiology of Alzheimer disease, LRP1 has a role in macrophage migration and that it is critical for internalization of integrin alpha-M/beta-2.

Anti-LRP1 Picoband™ Antibody Images

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Anti-LRP1 Picoband™ Antibody
Anti-LRP1 Picoband antibody, PB9143-1.jpg
All lanes: Anti LRP1 (PB9143) at 0.5ug/ml
WB: Recombinant Human LRP1 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to lrp1 antibody, cd91 antibody, lrp antibody