|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-MADCAM-1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Mucosal addressin cell adhesion molecule 1(MADCAM1) detection. Tested with WB, IHC-P, IHC-F in Human.|
|Cite This Product||Anti-MADCAM-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2083)|
|Replacement Item||This antibody may replace the following items: sc-16004|sc-19604|sc-28645|sc-365934|sc-514599 from Santa Cruz Biotechnology.|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human MAdCAM1(173-190aa EEPQGDEDVLFRVTERWR).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mucosal addressin cell adhesion molecule 1|
|Molecular Weight||40155 MW|
|Protein Function||Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L- selectin binding.|
|Tissue Specificity||Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. .|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Mucosal addressin cell adhesion molecule 1;MAdCAM-1;hMAdCAM-1;MADCAM1;|
|Research Areas|||cardiovascular|angiogenesis|adhesion / ecm|integrins| immunology|innate immunity|macrophage / inflamm.| signal transduction|cytoskeleton / ecm|cell adhesion| neuroscience|cell adhesion proteins|receptors|beta integrins||
Background for Mucosal addressin cell adhesion molecule 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MADCAM-1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MADCAM-1 Antibody Images
Click the images to enlarge.
IHC(P): Human Appendicitis Tissue
All lanes: Anti MAdCAM1 (PA2083) at 0.5ug/ml
WB: HELA Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
IHC(F): Human Placenta Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,