Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody

MAGE 1 antibody

Boster Bio Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody (Catalog # M03570). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rat, Dog.

Product Info Summary

SKU: M03570
Size: 100ug/vial
Reactive Species: Dog, Human, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC

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Product Name

Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody

View all MAGE 1 Antibodies

SKU/Catalog Number

M03570

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody (Catalog # M03570). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rat, Dog.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03570)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM282

Isotype

IgG1, kappa

Immunogen

Human MAGE-A1 full length recombinant protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03570 is reactive to MAGEA1 in Dog, Human, Rat

Applications

M03570 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

34.342kDa

Background of MAGE 1

Recognizes a protein of 42-46kDa, identified as MAGE-1. This monoclonal antibody does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For MAGEA1 (Source: Uniprot.org, NCBI)

Gene Name

MAGEA1

Full Name

Melanoma-associated antigen 1

Weight

34.342kDa

Alternative Names

Antigen MZ2-E; Cancer/testis antigen 1.1; cancer/testis antigen family 1, member 1; CT1.1melanoma antigen family A 1; MAGE-1 antigen; MAGE1A; MAGE1melanoma antigen MAGE-1; melanoma antigen family A, 1 (directs expression of antigen MZ2-E); melanoma-associated antigen 1; melanoma-associated antigen MZ2-E; MGC9326 MAGEA1 CT1.1, MAGE1 MAGE family member A1 melanoma-associated antigen 1|MAGE-1 antigen|antigen MZ2-E|cancer/testis antigen 1.1|cancer/testis antigen family 1, member 1|melanoma antigen MAGE-1|melanoma antigen family A 1|melanoma antigen family A, 1 (directs expression of antigen MZ2-E)|melanoma antigen family A1|melanoma-associated antigen MZ2-E

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAGEA1, check out the MAGEA1 Infographic

MAGEA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAGEA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody

Question

We are currently using anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says canine, human, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

The anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody (M03570) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-10

Question

I have a question about product M03570, anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03570 anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-19

Question

See below the WB image, lot number and protocol we used for testis using anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-07

Question

Is a blocking peptide available for product anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody (M03570)?

Verified Customer

Verified customer

Asked: 2019-04-11

Answer

We do provide the blocking peptide for product anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody (M03570). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-04-11

Question

I see that the anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570 works with IHC, what is the protocol used to produce the result images on the product page?

J. Bhatt

Verified customer

Asked: 2018-07-09

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-09

Question

Will M03570 anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Taylor

Verified customer

Asked: 2015-10-07

Answer

It shows on the product datasheet, M03570 anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-10-07

Question

Will anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570 work for IHC with testis?

N. Mitchell

Verified customer

Asked: 2014-08-13

Answer

According to the expression profile of testis, MAGEA1 is highly expressed in testis. So, it is likely that anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570 will work for IHC with testis.

Boster Scientific Support

Answered: 2014-08-13

Order DetailsPrice
M03570-100ug

100 μg

$468

Free Secondary Antibody

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M03570
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

Troubleshooting

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