Data & Images
|Product Name||Anti-MALT1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Mucosa-associated lymphoid tissue lymphoma translocation protein 1(MALT1) detection. Tested with WB in Human.|
|Cite This Product||Anti-MALT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1920)|
|Replacement Item||This antibody may replace the following items: sc-130494|sc-33952|sc-33953|sc-28246|sc-20552|sc-20553|sc-46677 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MALT1(809-824aa DEIPFSFSDRLRISEK).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mucosa-associated lymphoid tissue lymphoma translocation protein 1|
|Molecular Weight||92272 MW|
|Protein Function||Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion. .|
|Tissue Specificity||Highly expressed in peripheral blood mononuclear cells. Detected at lower levels in bone marrow, thymus and lymph node, and at very low levels in colon and lung.|
|Sequence Similarities||Belongs to the peptidase C14B family.|
|Subcellular Localization||Cytoplasm, perinuclear region . Nucleus . Shuttles between the nucleus and cytoplasm. Found in perinuclear structures together with BCL10.|
|Alternative Names||Mucosa-associated lymphoid tissue lymphoma translocation protein 1;3.4.22.-;MALT lymphoma-associated translocation;Paracaspase;MALT1;MLT;|
|Research Areas|||cell biology|apoptosis|intracellular|caspases etc|caspases| signal transduction|signaling pathway|nuclear signaling|nfkb pathway||
Background for Mucosa-associated lymphoid tissue lymphoma translocation protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MALT1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MALT1 Antibody Images
Click the images to enlarge.
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,