Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT)

MAPK3 antibody

Boster Bio Anti-Phospho-MAP Kinase, Activated (Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) catalog # MA1055. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Yeast. Cited in 7 publication(s).

Product Info Summary

SKU: MA1055
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat, Yeast
Host: Mouse
Application: IHC, ICC, WB

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Product Name

Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT)

View all MAPK3 Antibodies

SKU/Catalog Number

MA1055

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Phospho-MAP Kinase, Activated (Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) catalog # MA1055. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Yeast.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1055)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

MAPK-YT

Isotype

Mouse IgG1

Immunogen

A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1055 is reactive to MAPK3 in Human, Mouse, Rat, Yeast

Applications

MA1055 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

43.136kDa

Background of MAPK3

In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein (MAP) kinase pathways. The central core of each MAP kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, yeast, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, yeast, -
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, yeast

Validation Images & Assay Conditions

Gene/Protein Information For MAPK3 (Source: Uniprot.org, NCBI)

Gene Name

MAPK3

Full Name

Mitogen-activated protein kinase 3

Weight

43.136kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24 MAPK3 ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK mitogen-activated protein kinase 3 mitogen-activated protein kinase 3|MAPK 1|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1|insulin-stimulated MAP2 kinase|microtubule-associated protein 2 kinase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAPK3, check out the MAPK3 Infographic

MAPK3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAPK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

MA1055 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Mehrdoost, S., Yaghmaei, P., Jafary, H., Ebrahim-Habibi, A. (2021). The therapeutic effects of berberine plus sitagliptin in a rat model of fatty liver disease. Iranian Journal of Basic Medical Sciences, 24(4), 451-459. doi: 10.22038/ijbms.2021.52239.11822
Species: Rat
MA1055 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Huanyue Cui,Xueying Liu,Jin Zhang,Ke Zhang,Dahong Yao,Shi Dong,Shushu Feng,Lu Yang, Yuyao Li,Hangyu Wang,Jian Huang,Jinhui Wang,"Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways",Evidence-Based Complementary and Alternative Medicine,vol. 2021,Article ID 6103158,18 pages,2021.https://doi.org/10.1155/2021/6103158
Species: Human,Rat
MA1055 usage in article: APP: WB, SAMPLE:LUNG TISSUE, DILUTION:1:200

Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
MA1055 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA

Ji F,Wang Y,Yuan J,Wu Q,Wang J,Liu D.The potential role of stromal cell-derived factor-1α/CXCR4/CXCR7 axis in adipose-derived mesenchymal stem cells.J Cell Physiol.2020 Apr;235(4):3548-3557.doi:10.1002/jcp.29243.Epub 2019 Sep 30.PMID:31566725.
Species: Rat
MA1055 usage in article: APP:IF, SAMPLE:ADSCS, DILUTION:1:400

Wang C,Wang J,Liu X,Han Z,Aimin Jiang,Wei Z,Yang Z.Cl-amidine attenuates lipopolysaccharide-induced mouse mastitis by inhibiting NF-κB, MAPK, NLRP3 signaling pathway and neutrophils extracellular traps release.Microb Pathog.2020 Sep 24;149:104530.doi:10.1
Species: Mouse

Upregulation of long noncoding rna ccaT1-l promotes epithelial%u2013mesenchymal transition in gastric adenocarcinoma

Li Xl, Chen Xq, Zhang Mn, Chen N, Nie L, Xu M, Gong J, Shen Pf, Su Zz, Weng X, Tan Jy, Zhao T, Zeng H, Zhou Q. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3871-81. Ecollection 2015. Sox9 Was Involved In Tkis Resistance In Renal Cell Carcinoma Via Raf/M...

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Order DetailsPrice
MA1055

100μg

$370
MA1055-10ug

10μg sample (liquid)

$99
MA1055-Biotin

100 μg Biotin conjugated

$570
MA1055-Cy3

100 μg Cy3 conjugated

$570
MA1055-Dylight488

100 μg Dylight488 conjugated

$570
MA1055-Dylight550

100 μg Dylight550 conjugated

$570
MA1055-Dylight594

100 μg Dylight594 conjugated

$570
MA1055-FITC

100 μg FITC conjugated

$570
MA1055-HRP

100 μg HRP conjugated

$570
MA1055-APC

100 μg APC conjugated

$670
MA1055-PE

100 μg PE conjugated

$670
MA1055-carrier-free

Carrier Free

$370

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