|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (Monoclonal, MAPK-YT)|
|Description||Mouse IgG monoclonal antibody for MAP Kinase, Activated (Diphosphorylated ERK-1&2), mitogen-activated protein kinase 1; mitogen-activated protein kinase 3 (MAPK1; MAPK3 ) detection. Tested with WB, IHC-P, ICC in Human;mouse;rat;yeast. No cross reactivity with other proteins.|
|Cite This Product||Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (Monoclonal, MAPK-YT) MAPK-YT (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1055)|
|Replacement Item||This antibody may replace the following items: sc-101760|sc-101761|sc-135900|sc-136288|sc-136521|sc-137996|sc-137997|sc-138781|sc-138782|sc-153|sc-154|sc-154-G|sc-1647|sc-16981-R|sc-16982|sc-16982-R|sc-23759-R|sc-271269|sc-271270|sc-271291|sc-271451|sc-271458|sc-292838|sc-376852|sc-514302|sc-65981|sc-7383|sc-7976|sc-7976-R|sc-81457|sc-81458|sc-81459|sc-81492|sc-84436|sc-84437|sc-84819|sc-84821|sc-93|sc-93-G|sc-94|sc-94-G from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat, Yeast|
|Predicted Species||Bovine, Monkey|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3|
|Molecular Weight||43081 MW|
|Protein Function||Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.|
|Tissue Specificity||Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung.|
|Sequence Similarities||Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.|
|Subcellular Localization||Cytoplasm . Nucleus. Autophosphorylation at Thr-207 promotes nuclear localization. PEA15-binding redirects the biological outcome of MAPK3 kinase- signaling by sequestering MAPK3 into the cytoplasm. .|
|Alternative Names||Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;184.108.40.206;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;MNK1;Microtubule-associated protein 2 kinase;p44-ERK1;Mapk3;Erk1, Prkm3;|
|Research Areas||neuroscience|neurology process|neurodegenerative disease|alzheimer's disease|tangles & tau|signal transduction|protein phosphorylation|ser / thr kinases|mapk pathway|stem cells|signaling pathways|tgf beta|cytoplasmic|kits/ lysates/ other|kits|elisa kits|signal transduction proteins elisa kits|neuroscience target elisa kits||
Background for Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Antibody (Monoclonal, MAPK-YT) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.4-1μg/ml, Human, mouse, rat, yeast, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rat, yeast, -
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, yeast
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.