Anti-MAP3K8 Antibody Picoband™

MAP3K8/Tpl2/COT antibody

Boster Bio Anti-MAP3K8 Antibody Picoband™ catalog # PB10007. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: PB10007
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-MAP3K8 Antibody Picoband™

View all MAP3K8/Tpl2/COT Antibodies

SKU/Catalog Number

PB10007

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MAP3K8 Antibody Picoband™ catalog # PB10007. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MAP3K8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10007)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MAP3K8 recombinant protein (Position: D9-L193). Human MAP3K8 shares 89.7% and 90.3% amino acid (aa) sequence identity with mouse and rat MAP3K8, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10007 is reactive to MAP3K8 in Human, Rat

Applications

PB10007 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

52.925kDa

Background of MAP3K8/Tpl2/COT

Mitogen-activated protein kinase kinase kinase 8 is an enzyme that in humans is encoded by the MAP3K8 gene. This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. Additionally, this protein was found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For MAP3K8 (Source: Uniprot.org, NCBI)

Gene Name

MAP3K8

Full Name

Mitogen-activated protein kinase kinase kinase 8

Weight

52.925kDa

Superfamily

protein kinase superfamily

Alternative Names

Cancer Osaka thyroid oncogene; c-COT; cot (cancer Osaka thyroid) oncogene; COT; COTMEKK8; EC 2.7.11.25; EST; ESTF; ESTFLJ10486; Ewing sarcoma transformant; MAP3K8; mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TPL2; Tpl-2; Tumor progression locus 2; tumor progression locus-2 MAP3K8 AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT mitogen-activated protein kinase kinase kinase 8 mitogen-activated protein kinase kinase kinase 8|Ewing sarcoma transformant|augmented in rheumatoid arthritis 2|cot (cancer Osaka thyroid) oncogene|proto-oncogene c-Cot|proto-oncogene serine/threoine protein kinase|tumor progression locus 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MAP3K8, check out the MAP3K8 Infographic

MAP3K8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MAP3K8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-MAP3K8 Antibody Picoband™

Question

We are currently using anti-MAP3K8 antibody PB10007 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?

M. Walker

Verified customer

Asked: 2017-10-27

Answer

The anti-MAP3K8 antibody (PB10007) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-27

Order DetailsPrice
PB10007

100μg

$370
PB10007-10ug

10μg sample (liquid)

$99
PB10007-Biotin

100 μg Biotin conjugated

$570
PB10007-Cy3

100 μg Cy3 conjugated

$570
PB10007-Dylight488

100 μg Dylight488 conjugated

$570
PB10007-Dylight550

100 μg Dylight550 conjugated

$570
PB10007-Dylight594

100 μg Dylight594 conjugated

$570
PB10007-FITC

100 μg FITC conjugated

$570
PB10007-HRP

100 μg HRP conjugated

$570
PB10007-APC

100 μg APC conjugated

$670
PB10007-PE

100 μg PE conjugated

$670
PB10007-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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