Data & Images
|Product Name||Anti-MBD1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 1(MBD1) detection. Tested with WB in Human;Mouse.|
|Cite This Product||Anti-MBD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2041)|
|Replacement Item||This antibody may replace the following items: sc-9394|sc-10751|sc-25261|sc-55473|sc-52901|sc-9395 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human MBD1(366-381aa NQKRQKCRWRQCLQFA), identical to the related mouse sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Methyl-CpG-binding domain protein 1|
|Molecular Weight||66607 MW|
|Protein Function||Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters. .|
|Tissue Specificity||Widely expressed. .|
|Sequence Similarities||Contains 3 CXXC-type zinc fingers.|
|Subcellular Localization||Nucleus . Nucleus matrix . Nucleus speckle. Chromosome. Colocalizes with the Ten-1 ICD form of TENM1 in foci associated with the nuclear matrix (By similarity). Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes. .|
|Alternative Names||Methyl-CpG-binding domain protein 1;CXXC-type zinc finger protein 3;Methyl-CpG-binding protein MBD1;Protein containing methyl-CpG-binding domain 1;MBD1;CXXC3, PCM1;|
|Research Areas|||epigenetics and nuclear signaling|dna methylation|methylated dna binding||
Background for Methyl-CpG-binding domain protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MBD1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MBD1 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human MBD1, 38.2KD (162aa tag+ R283-A463)
Lane 1: Recombinant Human MBD1 Protein 10ng
Lane 2: Recombinant Human MBD1 Protein 5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,