Anti-MCAK/KIF2C Antibody Picoband™

KIF2C antibody

Boster Bio Anti-MCAK/KIF2C Antibody Picoband™ catalog # PB9230. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9230
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-MCAK/KIF2C Antibody Picoband™

View all KIF2C Antibodies

SKU/Catalog Number

PB9230

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MCAK/KIF2C Antibody Picoband™ catalog # PB9230. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MCAK/KIF2C Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9230)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9230 is reactive to KIF2C in Human, Mouse, Rat

Applications

PB9230 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

81 kDa

Calculated molecular weight

81.313kDa

Background of KIF2C

Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. KIF2C uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For KIF2C (Source: Uniprot.org, NCBI)

Gene Name

KIF2C

Full Name

Kinesin-like protein KIF2C

Weight

81.313kDa

Superfamily

TRAFAC class myosin-kinesin ATPase superfamily

Alternative Names

kinesin family member 2C; kinesin-like 6 (mitotic centromere-associated kinesin); Kinesin-like protein 6; kinesin-like protein KIF2C; MCAKKNSL6kinesin-like 6; Mitotic centromere-associated kinesin KIF2C CT139, KNSL6, MCAK kinesin family member 2C kinesin-like protein KIF2C|kinesin-like protein 6|mitotic centromere-associated kinesin|testis tissue sperm-binding protein Li 68n

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KIF2C, check out the KIF2C Infographic

KIF2C infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KIF2C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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8 Customer Q&As for Anti-MCAK/KIF2C Antibody Picoband™

Question

Does PB9230 anti-MCAK/KIF2C antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Taylor

Verified customer

Asked: 2020-01-16

Answer

As indicated on the product datasheet, PB9230 anti-MCAK/KIF2C antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-16

Question

Is there a BSA free version of anti-MCAK/KIF2C antibody PB9230 available?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MCAK/KIF2C antibody PB9230 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-19

Question

We are currently using anti-MCAK/KIF2C antibody PB9230 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

The anti-MCAK/KIF2C antibody (PB9230) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-05

Question

What DAB substrate was used for the images for PB9230?

Verified customer

Asked: 2019-06-11

Answer

For the Anti-MCAK/KIF2C Antibody Picoband™ (PB9230), we used AR1022 to developing the images. https://www.bosterbio.com/dab-chromogenic-kit-brown-ar1022-boster.html

Boster Scientific Support

Answered: 2019-06-11

Question

Will anti-MCAK/KIF2C antibody PB9230 work on pig IHC with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

Our lab technicians have not validated anti-MCAK/KIF2C antibody PB9230 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MCAK/KIF2C antibody PB9230 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-10

Question

Would anti-MCAK/KIF2C antibody PB9230 work for IHC with testis?

Verified Customer

Verified customer

Asked: 2017-10-05

Answer

According to the expression profile of testis, KIF2C is highly expressed in testis. So, it is likely that anti-MCAK/KIF2C antibody PB9230 will work for IHC with testis.

Boster Scientific Support

Answered: 2017-10-05

Question

Is a blocking peptide available for product anti-MCAK/KIF2C antibody (PB9230)?

K. Jones

Verified customer

Asked: 2015-09-09

Answer

We do provide the blocking peptide for product anti-MCAK/KIF2C antibody (PB9230). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-09-09

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-MCAK/KIF2C antibody PB9230. Let me know if you need anything else.

P. Anderson

Verified customer

Asked: 2014-07-09

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-07-09

Order DetailsPrice
PB9230

100μg

$370
PB9230-10ug

10μg sample (liquid)

$99
PB9230-Biotin

100 μg Biotin conjugated

$570
PB9230-Cy3

100 μg Cy3 conjugated

$570
PB9230-Dylight488

100 μg Dylight488 conjugated

$570
PB9230-Dylight550

100 μg Dylight550 conjugated

$570
PB9230-Dylight594

100 μg Dylight594 conjugated

$570
PB9230-FITC

100 μg FITC conjugated

$570
PB9230-HRP

100 μg HRP conjugated

$570
PB9230-APC

100 μg APC conjugated

$670
PB9230-PE

100 μg PE conjugated

$670
PB9230-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9230
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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