Product Info Summary
SKU: | PB9230 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MCAK/KIF2C Antibody Picoband™
SKU/Catalog Number
PB9230
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCAK/KIF2C Antibody Picoband™ catalog # PB9230. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCAK/KIF2C Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9230)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MCAK recombinant protein (Position: G531-Q725). Human MCAK shares 87% amino acid (aa) sequence identity with both mouse and rat MCAK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9230 is reactive to KIF2C in Human, Mouse, Rat
Applications
PB9230 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
81 kDa
Calculated molecular weight
81.313kDa
Background of KIF2C
Kinesin-like protein KIF2C is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. KIF2C uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-MCAK antibody, PB9230, Western blotting
All lanes: Anti MCAK (PB9230) at 0.5ug/ml
WB: Recombinant Human MCAK Protein 0.5ng
Predicted bind size: 48KD
Observed bind size: 48KD
Click image to see more details
Anti-MCAK antibody, PB9230, Western blotting
All lanes: Anti MCAK (PB9230) at 0.5ug/ml
Lane 1: Mouse Testis Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: MM453 Whole Cell Lysate at 40ug
Lane 5: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 81KD
Observed bind size: 81KD
Click image to see more details
Anti-MCAK antibody, PB9230, IHC(P)
IHC(P): Mouse Testis Tissue
Click image to see more details
Anti-MCAK antibody, PB9230, IHC(P)
IHC(P): Rat Testis Tissue
Click image to see more details
Anti-MCAK antibody, PB9230, IHC(P)
IHC(P): Rat Thymus Tissue
Click image to see more details
Figure 6. IF analysis of MCAK using anti-MCAK antibody (PB9230).
MCAK was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCAK Antibody (PB9230) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. Flow Cytometry analysis of K562 cells using anti-MCAK antibody (PB9230).
Overlay histogram showing K562 cells stained with PB9230 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAK Antibody (PB9230,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For KIF2C (Source: Uniprot.org, NCBI)
Gene Name
KIF2C
Full Name
Kinesin-like protein KIF2C
Weight
81.313kDa
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
kinesin family member 2C; kinesin-like 6 (mitotic centromere-associated kinesin); Kinesin-like protein 6; kinesin-like protein KIF2C; MCAKKNSL6kinesin-like 6; Mitotic centromere-associated kinesin KIF2C CT139, KNSL6, MCAK kinesin family member 2C kinesin-like protein KIF2C|kinesin-like protein 6|mitotic centromere-associated kinesin|testis tissue sperm-binding protein Li 68n
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIF2C, check out the KIF2C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIF2C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCAK/KIF2C Antibody Picoband™ (PB9230)
Hello CJ!
No publications found for PB9230
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MCAK/KIF2C Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-MCAK/KIF2C Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
8 Customer Q&As for Anti-MCAK/KIF2C Antibody Picoband™
Question
Does PB9230 anti-MCAK/KIF2C antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Taylor
Verified customer
Asked: 2020-01-16
Answer
As indicated on the product datasheet, PB9230 anti-MCAK/KIF2C antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-16
Question
Is there a BSA free version of anti-MCAK/KIF2C antibody PB9230 available?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MCAK/KIF2C antibody PB9230 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-19
Question
We are currently using anti-MCAK/KIF2C antibody PB9230 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The anti-MCAK/KIF2C antibody (PB9230) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-05
Question
What DAB substrate was used for the images for PB9230?
Verified customer
Asked: 2019-06-11
Answer
For the Anti-MCAK/KIF2C Antibody Picoband™ (PB9230), we used AR1022 to developing the images. https://www.bosterbio.com/dab-chromogenic-kit-brown-ar1022-boster.html
Boster Scientific Support
Answered: 2019-06-11
Question
Will anti-MCAK/KIF2C antibody PB9230 work on pig IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
Our lab technicians have not validated anti-MCAK/KIF2C antibody PB9230 on pig. You can run a BLAST between pig and the immunogen sequence of anti-MCAK/KIF2C antibody PB9230 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-10
Question
Would anti-MCAK/KIF2C antibody PB9230 work for IHC with testis?
Verified Customer
Verified customer
Asked: 2017-10-05
Answer
According to the expression profile of testis, KIF2C is highly expressed in testis. So, it is likely that anti-MCAK/KIF2C antibody PB9230 will work for IHC with testis.
Boster Scientific Support
Answered: 2017-10-05
Question
Is a blocking peptide available for product anti-MCAK/KIF2C antibody (PB9230)?
K. Jones
Verified customer
Asked: 2015-09-09
Answer
We do provide the blocking peptide for product anti-MCAK/KIF2C antibody (PB9230). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-09-09
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-MCAK/KIF2C antibody PB9230. Let me know if you need anything else.
P. Anderson
Verified customer
Asked: 2014-07-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-09