Data & Images
|Product Name||Anti-MCK10 Antibody|
|Description||Rabbit IgG polyclonal antibody for Epithelial discoidin domain-containing receptor 1(DDR1) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-MCK10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1878)|
|Replacement Item||This antibody may replace the following items: sc-532|sc-7553|sc-8988|sc-374618|sc-21790|sc-390268|sc-532-G from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MCK10(898-913aa FSQLHRFLAEDALNTV), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Epithelial discoidin domain-containing receptor 1|
|Molecular Weight||101128 MW|
|Protein Function||Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. .|
|Tissue Specificity||Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.|
|Subcellular Localization||Isoform 1: Cell membrane; Single-pass type I membrane protein.|
|Alternative Names||Epithelial discoidin domain-containing receptor 1;Epithelial discoidin domain receptor 1;188.8.131.52;CD167 antigen-like family member A;Cell adhesion kinase;Discoidin receptor tyrosine kinase;HGK2;Mammary carcinoma kinase 10;MCK-10;Protein-tyrosine kinase 3A;Protein-tyrosine kinase RTK-6;TRK E;Tyrosine kinase DDR;Tyrosine-protein kinase CAK;CD167a;DDR1;CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE;|
|Research Areas|||neuroscience|neurotransmission|receptors / channels|more ion channels| cancer|signal transduction|protein phosphorylation|tyrosine kinases|receptor tyrosine kinases| cardiovascular|heart|cardiogenesis|cardiomyocyte precursors||
Background for Epithelial discoidin domain-containing receptor 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MCK10 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MCK10 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant human DDR1, 45.8KD (162aa tag+F669-V913)
Lane 1: Recombinant Human DDR1 Protein 10ng
Lane 2: Recombinant Human DDR1 Protein 5ng
Lane 3: Recombinant Human DDR1 Protein 2.5ng
Lane 4: Recombinant Human DDR1 Protein 1.25ng
Lane 1: A431 Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: HELA Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,