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SKU:PB9132
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-MCL1 Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Induced myeloid leukemia cell differentiation protein Mcl-1(MCL1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-MCL1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9132)
Replacement Item This antibody may replace the following items: sc-12756|sc-20679|sc-377487|sc-53951|sc-53971|sc-56152|sc-56427|sc-69838|sc-69839|sc-69840|sc-69841|sc-74436|sc-74437|sc-819|sc-958 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human MCL1 recombinant protein (Position: M1-R350). Human MCL1 shares 82% and 78% amino acid (aa) sequences identity with mouse and rat MCL1, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MCL1
Protein Name Induced myeloid leukemia cell differentiation protein Mcl-1
Molecular Weight 37337 MW
Protein Function Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. .
Sequence Similarities Belongs to the Bcl-2 family.
Subcellular Localization Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.
Uniprot ID Q07820
Alternative Names Induced myeloid leukemia cell differentiation protein Mcl-1;Bcl-2-like protein 3;Bcl2-L-3;Bcl-2-related protein EAT/mcl1;mcl1/EAT;MCL1;BCL2L3;
Research Areas |cell biology|apoptosis|intracellular|bcl2 family| cancer|invasion/microenvironment|bcl 2 family| metabolism|pathways and processes|metabolism processes|cell death|apoptotic markers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Induced myeloid leukemia cell differentiation protein Mcl-1

MCL1, also known as myeloid cell leukemia 1, is a protein that in humans is encoded the MCL1 gene. It is mapped to 1q21.3. MCL1 is a potent multidomain antiapoptotic protein of the BCL2 family that heterodimerizes with other BCL2 family members to protect against apoptotic cell death. MCL1 as an attractive candidate for regulation of hematopoietic stem cell homeostasis that is highly expressed in hematopoietic stem cells and regulated by growth factor signals. MCL1 is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. During mitotic arrest, MCL1 protein levels decline markedly, through a posttranslational mechanism, potentiating cell death. Phosphorylation of MCL1 directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex.

Anti-MCL1 Picoband™ Antibody Images

Click the images to enlarge.

Anti-MCL1 Picoband™ Antibody
Anti-MCL1 Picoband antibody, PB9132-1.jpg
All lanes: Anti MCL1 (PB9132) at 0.5ug/ml
WB: Recombinant Human MCL1 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-MCL1 Picoband™ Antibody
Anti-MCL1 Picoband antibody, PB9132-2.jpg
All lanes: Anti MCL1 (PB9132) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
Anti-MCL1 Picoband™ Antibody
Anti-MCL1 Picoband antibody, PB9132-3.JPG
IHC(P): Rat Intestine Tissue
Anti-MCL1 Picoband™ Antibody
Anti-MCL1 Picoband antibody, PB9132-4.JPG
IHC(P): Human Intestinal Cancer Tissue
Anti-MCL1 Picoband™ Antibody
Anti-MCL1 Picoband antibody, PB9132-5.JPG
IHC(P): Mouse Intestine Tissue
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Publications

Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mcl 1 antibody, mcl1 antibody