|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-MCM2 Antibody|
|Description||Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM2(MCM2) detection. Tested with WB, IHC-P, ICC in Mouse;Rat.|
|Cite This Product||Anti-MCM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1711)|
|Replacement Item||This antibody may replace the following items: sc-9839|sc-10771|sc-373702|sc-28551|sc-56322|sc-73572|sc-56321|sc-6680 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2(884-904aa DLFKFNKFSRDLKRKLILQQF), different from the related rat sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||DNA replication licensing factor MCM2|
|Molecular Weight||102078 MW|
|Protein Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.|
|Sequence Similarities||Belongs to the MCM family.|
|Subcellular Localization||Nucleus .|
|Alternative Names||DNA replication licensing factor MCM2;126.96.36.199;Minichromosome maintenance protein 2 homolog;Nuclear protein BM28;Mcm2;Bm28, Cdcl1, Kiaa0030, Mcmd2;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|dna synthesis||
Background for DNA replication licensing factor MCM2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MCM2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Mouse, Rat|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MCM2 Antibody Images
Click the images to enlarge.
IHC(P): Rat Intestinal Lymphocyte Tissue
IHC(P): Mouse Intestine Tissue
All lanes: Anti MCM2 (PA1711) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouse Intestine Tissue Lysate at 50ug
Lane 4: Mouse Testis Tissue Lysate at 50ug
Lane 5: Mouse Ovary Tissue Lysate at 50ug
Lane 6: Mouse Kidney Tissue Lysate at 50ug
Predicted bind size: 102KD
Observed bind size: 102KD
ICC: HEPA Cell
ICC: NIH3T3 Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,