|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-MCM3 Antibody|
|Description||Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM3(MCM3) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-MCM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1651)|
|Replacement Item||This antibody may replace the following items: sc-9850|sc-9849|sc-365616|sc-292857|sc-390480|sc-6682|sc-6683 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3(13-27aa REAQRDYLDFLDDEE), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||DNA replication licensing factor MCM3|
|Molecular Weight||90981 MW|
|Protein Function||Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.|
|Sequence Similarities||Belongs to the MCM family.|
|Alternative Names||DNA replication licensing factor MCM3;188.8.131.52;DNA polymerase alpha holoenzyme-associated protein P1;P1-MCM3;RLF subunit beta;p102;MCM3;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|dna synthesis| epigenetics and nuclear signaling|chip'ing antibodies||
Background for DNA replication licensing factor MCM3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MCM3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MCM3 Antibody Images
Click the images to enlarge.
All lanes: Anti MCM3 (PA1651) at 0.5ug/ml
Lane 1: NIH3T3 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
IHC(P): Human Lung Cancer Tissue
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
IHC(P): Mouse Intestine Tissue
ICC: HELA Cell
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,