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SKU:MA1058
Clonality:Monoclonal
Clone Number:DCS-141
Application:ICC, WB
Price: $200.00
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Overview

Product Name Anti-Mcm7 Antibody (Monoclonal, DCS-141)
SKU/Catalog Number MA1058
Description Mouse IgG monoclonal antibody for Mcm7, minichromosome maintenance complex component 7 (MCM7) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-Mcm7 Antibody (Monoclonal, DCS-141) DCS-141 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1058)
Replacement Item This antibody may replace the following items: sc-9794|sc-56429|sc-9847|sc-374403|sc-22782|sc-65469|sc-65475|sc-56324|sc-71550|sc-46687|sc-9966|sc-6688 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human Mcm7.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number DCS-141
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Mcm7
Protein Name DNA replication licensing factor MCM7
Molecular Weight 81063 MW
Protein Function MCM7: Acts as component of the MCM2-7 complex (MCM complex)which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage.
Sequence Similarities Belongs to the MCM family.
Subcellular Localization Nucleus .
Uniprot ID Q6AYN8
Alternative Names DNA helicase ;3.6.4.12 ;Mcm7 ;rCG_55961 ;
Research Areas |epigenetics and nuclear signaling|dna / rna|dna synthesis|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for DNA replication licensing factor MCM7

Initiation of DNA replication is a complex process involving the concerted action of many proteins. The chromosome 7-linked gene referred to by Nakatsuru et al. as MCM2 has been designated MCM7. The MCM2-7 complex is comprised of 6 subunits, MCM2 through MCM7, and is a ring-shaped heterohexameric ATPase involved in DNA replication. MCM7 is located in 7q21.3-q22.1.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.