Data & Images
|Product Name||Anti-Mcm7 Antibody (Monoclonal, DCS-141)|
|Description||Mouse IgG monoclonal antibody for Mcm7, minichromosome maintenance complex component 7 (MCM7) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Mcm7 Antibody (Monoclonal, DCS-141) DCS-141 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1058)|
|Replacement Item||This antibody may replace the following items: sc-9794|sc-56429|sc-9847|sc-374403|sc-22782|sc-65469|sc-65475|sc-56324|sc-71550|sc-46687|sc-9966|sc-6688 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant human Mcm7.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||DNA replication licensing factor MCM7|
|Molecular Weight||81063 MW|
|Protein Function||MCM7: Acts as component of the MCM2-7 complex (MCM complex)which is the putative replicative helicase essential for 'once percell cycle' DNA replication initiation and elongation ineukaryotic cells. The active ATPase sites in the MCM2-7 ring areformed through the interaction surfaces of two neighboringsubunits such that a critical structure of a conserved argininefinger motif is provided in trans relative to the ATP-binding siteof the Walker A box of the adjacent subunit. The six ATPase activesites, however, are likely to contribute differentially to thecomplex helicase activity. Required for S-phase checkpointactivation upon UV-induced damage.|
|Sequence Similarities||Belongs to the MCM family.|
|Subcellular Localization||Nucleus .|
|Alternative Names||DNA helicase ;22.214.171.124 ;Mcm7 ;rCG_55961 ;|
|Research Areas|||epigenetics and nuclear signaling|dna / rna|dna synthesis||
Background for DNA replication licensing factor MCM7
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Mcm7 Antibody (Monoclonal, DCS-141) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, -|
Western blot, 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.