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SKU:PB9030
Clonality:Polyclonal
Application:ELISA, WB
Price: $240.00
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Overview

Product Name Anti-CCL13/MCP4 Antibody
SKU/Catalog Number PB9030
Description Rabbit IgG polyclonal antibody for C-C motif chemokine 13(CCL13) detection. Tested with WB, ELISA in Human.
Cite This Product Anti-CCL13/MCP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9030)
Replacement Item This antibody may replace the following items: sc-271124|sc-28971|sc-377082|sc-9655|sc-9656 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application ELISA, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human MCP4 recombinant protein (Position: Q24-T98).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CCL13
Protein Name C-C motif chemokine 13
Molecular Weight 10986 MW
Protein Function Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
Tissue Specificity Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.
Sequence Similarities Belongs to the intercrine beta (chemokine CC) family.
Subcellular Localization Secreted.
Uniprot ID Q99616
Alternative Names C-C motif chemokine 13;CK-beta-10;Monocyte chemoattractant protein 4;Monocyte chemotactic protein 4;MCP-4;NCC-1;Small-inducible cytokine A13;C-C motif chemokine 13, long chain;C-C motif chemokine 13, medium chain;C-C motif chemokine 13, short chain;CCL13;MCP4, NCC1, SCYA13;
Research Areas |immunology|innate immunity|macrophage / inflamm.| immunology|chemokines|beta chemokines (cc)|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for C-C motif chemokine 13

Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Anti-CCL13/MCP4 Antibody Images

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Anti-CCL13/MCP4 Antibody
Anti-MCP4 Picoband antibody, PB9030-1.jpg
All lanes: Anti-MCP4(PB9030) at 0.5ug/ml
WB: Recombinant Human MCP4 Protein 0.5ng
Predicted bind size: 34KD
Observed bind size: 34KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.