Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human;Mouse;Rat. Cited in 6 publication(s).
Anti-MDM2 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
FAS / CD95 is a 36kDa transmembrane type I receptor, which belongs to the NF/NGF receptor super-family. It is a potent inducer of apoptosis in cells of the immune system upon interaction with its natural ligand, CD95L. Although the CD95/CD95L system could play a role in tumor regression, tumor cells seem to down-regulate CD95 expression as a mechanism of resistance to CD95L-induced killing by T lymphocytes and NK cells. The Fas antigen is expressed on the surface of various cell types, including activated T and B lymphocytes and T lymphoblastoid cell line. CD95 is also expressed in a broad panel of non-transformed cells outside the immune system.
|Product Name||Anti-MDM2 Antibody
See all MDM2/HDM2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for HDM2/MDM2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. HDM2/MDM2 information: Molecular Weight: 55233 MW; Subcellular Localization: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus; Tissue Specificity: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.|
|Cite This Product||Anti-MDM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1378)|
|Similar Products From
|Anti-MDM2 Antibody may replace the following items: sc 56154|sc 56430|sc 965|sc 813|sc 7918|sc 5304|sc 812|sc 13161|sc 965 X.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human MDM2(103-119aa RKIYTMIYRNLVVVNQQ), different from the related mouse sequence by three amino acids, and different from rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For MDM2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||4193|
|Species Of This Entry||Human|
|Protein Name||E3 ubiquitin-protein ligase Mdm2|
|Alternative Names||MDM2/HDM2|ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2|
|Post Tranlational Modifications||Phosphorylation; Ubiquitination; Cleavage; Acetylation; Methylation; Dephosphorylation; Sumoylation; Reduction; Deacetylation; Demethylation; Phosphorothioation; Oxidation; Deamination; Nitration; Biotinylation; Myristoylation; Nitrosylation; Ribosylation; Glycosylation; Hydroxylation|
|Subcellular Localization||Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.|
|Gene Location||12q15, on Chromasome 12, gene sequence: NC_000012.12 (68808149..68850686)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MDM2 (Source: Uniprot.org, NCBI)
|Protein Function||E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity).|
|Tissue Specificity||Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.|
|Research Areas||p53 Pathway|
|Related Diseases||Neoplasms; Malignant Neoplasms; Carcinoma; Cell Transformation, Neoplastic; Malignant Paraganglionic Neoplasm; Retinoblastoma; Sarcoma; Mammary Neoplasms; Malignant Neoplasm Of Breast; Leukemia; Neoplasm Metastasis; Carcinogenesis; Liposarcoma; Malignant Squamous Cell Neoplasm; Growth Arrest; Osteosarcoma; Lung Neoplasms; Lymphoma; Adenocarcinoma; Brain Neoplasms|
|Related Pathways||Cell Cycle; Cell Cycle Arrest; Cell Death; Cell Proliferation; Cell Growth; Localization; Pathogenesis; Dna Repair; Nuclear Export; Senescence; Induction Of Apoptosis; Translation; Methylation; Proteolysis; S Phase; Oncogenesis; Angiogenesis; Rna Interference; G1 Phase; Programmed Cell Death|
|Background||Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.|
|Scientific References||PMID: 1614537 by Oliner J.D., et al. Amplification of a gene encoding a p53-associated protein in human sarcomas.|
PMID: 8705862 by Sigalas I., et al. Alternatively spliced mdm2 transcripts with loss of p53 binding domain sequences: transforming ability and frequent detection in human cancer.
Our Boster Quality Guarantee for Anti-MDM2 Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Mouse Liver Tissue Lysate
Lane 2: Mouse Spleen Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Thymus Tissue Lysate
Lane 5: Mouse Ovary Tissue Lysate
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 6
|Xiao S, Zhou Y, Yi W, Luo G, Jiang B, Tian Q, Li Y, Xue M. Int J Oncol. 2015 Apr;46(4):1677-84. Doi: 10.3892/Ijo.2015.2873. Epub 2015 Feb 4. Fra-1 Is Downregulated In Cervical Cancer Tissues And Promotes Cervical Cancer Cell Apoptosis By P53 Signa...||PubMed ID 25651840|
|Liao S, Xiao S, Zhu G, Zheng D, He J, Pei Z, Li G, Zhou Y. Oncol Rep. 2014 Dec;32(6):2703-9. Doi: 10.3892/Or.2014.3537. Epub 2014 Oct 10. Cd38 Is Highly Expressed And Affects The Pi3K/Akt Signaling Pathway In Cervical Cancer.||PubMed ID 25310288|
|The Oncoprotein HBXIP Modulates the Feedback Loop of MDM2/p53 to Enhance the Growth of Breast Cancer||PubMed ID 26229107|
|Fra-1 is upregulated in lung cancer tissues and inhibits the apoptosis of lung cancer cells by the P53 signaling pathway||PubMed ID 26549498|
|Fra-1 is upregulated in gastric cancer tissues and affects the PI3K/Akt and p53 signaling pathway in gastric cancer||PubMed ID 26330014|
|Epigenetic repression of long non-coding RNA MEG3 mediated by DNMT1 represses the p53 pathway in gliomas||PubMed ID 26676363|
Order Product (PA1378)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.