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SKU:PA1378
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-MDM2/Hdm2 Antibody
SKU/Catalog Number PA1378
Description Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-MDM2/Hdm2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1378)
Replacement Item This antibody may replace the following items: sc-56154|sc-56430|sc-965|sc-813|sc-7918|sc-5304|sc-812|sc-13161|sc-965-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Chicken

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human MDM2(103-119aa RKIYTMIYRNLVVVNQQ), different from the related mouse sequence by three amino acids, and different from rat sequence by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MDM2
Protein Name E3 ubiquitin-protein ligase Mdm2
Molecular Weight 55233 MW
Protein Function E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. .
Tissue Specificity Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
Sequence Similarities Belongs to the MDM2/MDM4 family.
Subcellular Localization Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
Uniprot ID Q00987
Alternative Names E3 ubiquitin-protein ligase Mdm2;6.3.2.-;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2;MDM2;
Research Areas |cell biology|cell cycle|cell cycle inhibitors|p53| epigenetics and nuclear signaling|transcription|other factors| cancer|p53 pathway|oncoproteins/suppressors|oncoproteins|cell survival & death|ubiquitin & ubiquitin like modifiers|e3 ubiquitin ligases|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for E3 ubiquitin-protein ligase Mdm2

Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

Anti-MDM2/Hdm2 Antibody Images

Click the images to enlarge.

Anti-MDM2/Hdm2 Antibody
Anti-MDM2 antibody, PA1378, Western blotting
Lane 1: Mouse Liver Tissue Lysate
Lane 2: Mouse Spleen Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Thymus Tissue Lysate
Lane 5: Mouse Ovary Tissue Lysate
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Publications

Liao S, Xiao S, Zhu G, Zheng D, He J, Pei Z, Li G, Zhou Y. Oncol Rep. 2014 Dec;32(6):2703-9. Doi: 10.3892/Or.2014.3537. Epub 2014 Oct 10. Cd38 Is Highly Expressed And Affects The Pi3K/Akt Signaling Pathway In Cervical Cancer.
Xiao S, Zhou Y, Yi W, Luo G, Jiang B, Tian Q, Li Y, Xue M. Int J Oncol. 2015 Apr;46(4):1677-84. Doi: 10.3892/Ijo.2015.2873. Epub 2015 Feb 4. Fra-1 Is Downregulated In Cervical Cancer Tissues And Promotes Cervical Cancer Cell Apoptosis By P53 Signa...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mdm2 antibody, hdm2 antibody, mtbp antibody
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mdm2 antibody, hdm2 antibody, mtbp antibody