Anti-MED24/Trap100 Antibody

MED24 antibody

Boster Bio Anti-MED24/Trap100 Antibody (Catalog # A05987). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05987
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, WB

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Product Name

Anti-MED24/Trap100 Antibody

View all MED24 Antibodies

SKU/Catalog Number

A05987

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MED24/Trap100 Antibody (Catalog # A05987). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED24/Trap100 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05987)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MED24.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05987 is reactive to MED24 in Human, Mouse, Rat

Applications

A05987 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

110.305kDa

Background of MED24

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Yuan C.-X., Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998).
Rachez C., Genes Dev. 12:1787-1800(1998).
Nagase T., DNA Res. 2:167-174(1995).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For MED24 (Source: Uniprot.org, NCBI)

Gene Name

MED24

Full Name

Mediator of RNA polymerase II transcription subunit 24

Weight

110.305kDa

Superfamily

Mediator complex subunit 24 family

Alternative Names

cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa; CRSP100; DRIP100CRSP complex subunit 4; hTRAP100; KIAA0130CRSP4; mediator complex subunit 24ARC100; MGC8748; subunit 24 homolog; thyroid hormone receptor associated protein 4; Thyroid hormone receptor-associated protein 4; Thyroid hormone receptor-associated protein complex 100 kDa component; Trap100; TRAP100THRAP4; Vitamin D3 receptor-interacting protein complex 100 kDa component; vitamin D3 receptor-interacting protein complex component DRIP100 MED24 ARC100, CRSP100, CRSP4, DRIP100, MED5, THRAP4, TRAP100 mediator complex subunit 24 mediator of RNA polymerase II transcription subunit 24|CRSP complex subunit 4|activator-recruited cofactor 100 kDa component|cofactor required for Sp1 transcriptional activation subunit 4|cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa|mediator of RNA polymerase II transcription, subunit 24 homolog|thyroid hormone receptor-associated protein 4|thyroid hormone receptor-associated protein complex 100 kDa component|vitamin D3 receptor-interacting protein complex 100 kDa component|vitamin D3 receptor-interacting protein complex component DRIP100

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MED24, check out the MED24 Infographic

MED24 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MED24: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-MED24/Trap100 Antibody

Question

Here is the WB image, lot number and protocol we used for eye using anti-MED24/Trap100 antibody A05987. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-03

Question

I have a question about product A05987, anti-MED24/Trap100 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05987 anti-MED24/Trap100 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-13

Question

Does anti-MED24/Trap100 antibody A05987 work for WB with eye?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

According to the expression profile of eye, MED24 is highly expressed in eye. So, it is likely that anti-MED24/Trap100 antibody A05987 will work for WB with eye.

Boster Scientific Support

Answered: 2019-12-03

Question

I am interested in to test anti-MED24/Trap100 antibody A05987 on rat eye for research purposes, then I may be interested in using anti-MED24/Trap100 antibody A05987 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

The products we sell, including anti-MED24/Trap100 antibody A05987, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-28

Question

We are currently using anti-MED24/Trap100 antibody A05987 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

J. Lewis

Verified customer

Asked: 2017-07-14

Answer

The anti-MED24/Trap100 antibody (A05987) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-14

Question

I see that the anti-MED24/Trap100 antibody A05987 works with WB, what is the protocol used to produce the result images on the product page?

K. Johnson

Verified customer

Asked: 2013-01-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-01-07

Order DetailsPrice
A05987-100uL

100uL

$360

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