Product Info Summary
SKU: | A06467-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MED4 Antibody Picoband™
SKU/Catalog Number
A06467-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MED4 Antibody Picoband™ catalog # A06467-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MED4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06467-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human MED4 recombinant protein (Position: R27-K140).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06467-1 is reactive to MED4 in Human, Mouse, Rat
Applications
A06467-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
34 kDa
Calculated molecular weight
29.745kDa
Background of MED4
Mediator of RNA polymerase II transcription subunit 4, also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36), is a protein that in humans is encoded by the MED4 gene. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. The MED4 gene was mapped to chromosome 13 based on sequence similarity between the MED4 sequence and a genomic contig.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MED4 using anti-MED4 antibody (A06467-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MED4 antigen affinity purified polyclonal antibody (Catalog # A06467-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MED4 at approximately 34KD. The expected band size for MED4 is at 30KD.
Click image to see more details
Figure 2. IHC analysis of MED4 using anti-MED4 antibody (A06467-1).
MED4 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (A06467-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MED4 using anti-MED4 antibody (A06467-1).
MED4 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (A06467-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of MED4 using anti-MED4 antibody (A06467-1).
MED4 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (A06467-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of MED4 using anti-MED4 antibody (A06467-1).
MED4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (A06467-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of MED4 using anti-MED4 antibody (A06467-1).
MED4 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (A06467-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of MED4 using anti-MED4 antibody (A06467-1).
MED4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (A06467-1) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MED4 (Source: Uniprot.org, NCBI)
Gene Name
MED4
Full Name
Mediator of RNA polymerase II transcription subunit 4
Weight
29.745kDa
Superfamily
Mediator complex subunit 4 family
Alternative Names
Activator-recruited cofactor 36 kDa component; ARC36; DRIP36; DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae); FLJ10956; HSPC126; HSPC126vitamin D receptor interacting protein; MED4; mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit; subunit 4 homolog; TRAP36; VDRIP MED4 ARC36, DRIP36, HSPC126, TRAP36, VDRIP mediator complex subunit 4 mediator of RNA polymerase II transcription subunit 4|activator-recruited cofactor 36 kDa component|mediator, 34-kD subunit, homolog|vitamin D receptor-interacting protein, 36-kD|vitamin D3 receptor-interacting protein complex 36 kDa component
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MED4, check out the MED4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MED4 Antibody Picoband™ (A06467-1)
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No publications found for A06467-1
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Customer Q&As
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1 Customer Q&As for Anti-MED4 Antibody Picoband™
Question
We are currently using anti-MED4 antibody A06467-1 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
N. Patel
Verified customer
Asked: 2017-05-09
Answer
The anti-MED4 antibody (A06467-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-09