|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-MEF2A Antibody|
|Description||Rabbit IgG polyclonal antibody for Myocyte-specific enhancer factor 2A(MEF2A) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-MEF2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1823)|
|Replacement Item||This antibody may replace the following items: sc-101731|sc-101732|sc-10794|sc-10794-X|sc-13917|sc-13917-X|sc-17785|sc-17785-X|sc-313|sc-313-X|sc-55500 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2A(358-374aa MLSLGQVSAWQQHHLGQ), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Myocyte-specific enhancer factor 2A|
|Molecular Weight||54811 MW|
|Protein Function||Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle- specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter. .|
|Tissue Specificity||Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined. .|
|Sequence Similarities||Belongs to the MEF2 family.|
|Subcellular Localization||Nucleus .|
|Alternative Names||Myocyte-specific enhancer factor 2A;Serum response factor-like protein 1;MEF2A;MEF2;|
|Research Areas|||signal transduction|signaling pathway|calcium signaling|calmodulin pathway| neuroscience|neurology process|neurogenesis| epigenetics and nuclear signaling|transcription|other factors|domain families|developmental families|transcription factors| stem cells|mesenchymal stem cells|myogenesis| cardiovascular|heart|cardiogenesis|transcription factors/regulators|hypertrophy||
Background for Myocyte-specific enhancer factor 2A
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MEF2A Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MEF2A Antibody Images
Click the images to enlarge.
All lanes: Anti MEF2A (PA1823) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HT1080Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,