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SKU:PA1823
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-MEF2A Antibody
Description Rabbit IgG polyclonal antibody for Myocyte-specific enhancer factor 2A(MEF2A) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-MEF2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1823)
Replacement Item This antibody may replace the following items: sc-101731|sc-101732|sc-10794|sc-10794-X|sc-13917|sc-13917-X|sc-17785|sc-17785-X|sc-313|sc-313-X|sc-55500 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2A(358-374aa MLSLGQVSAWQQHHLGQ), different from the related mouse and rat sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MEF2A
Protein Name Myocyte-specific enhancer factor 2A
Molecular Weight 54811 MW
Protein Function Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle- specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter. .
Tissue Specificity Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined. .
Sequence Similarities Belongs to the MEF2 family.
Subcellular Localization Nucleus .
Uniprot ID Q02078
Alternative Names Myocyte-specific enhancer factor 2A;Serum response factor-like protein 1;MEF2A;MEF2;
Research Areas |signal transduction|signaling pathway|calcium signaling|calmodulin pathway| neuroscience|neurology process|neurogenesis| epigenetics and nuclear signaling|transcription|other factors|domain families|developmental families|transcription factors| stem cells|mesenchymal stem cells|myogenesis| cardiovascular|heart|cardiogenesis|transcription factors/regulators|hypertrophy|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Myocyte-specific enhancer factor 2A

MEF2A(myocyte enhancer factor 2A) also known as RSRFC4, RSRFC9,is a protein that in humans is encoded by the MEF2A gene. MEF2A is a transcription factor in the Mef2 family. Several alternative splice variants of MEF2A were identified that were predicted to encode different protein products. Using immunofluorescence, MEF2A protein was detected in the nuclei of skeletal and cardiac muscle cells. The MEF2A gene is mapped on 15q26.3. MEF2A may be involved in induction of muscle differentiation and MEF2C in maintenance of the differentiated state. Coimmunoprecipitation assays indicated that GEF bound both MEF2A and MEF2D in vitro, and MEF2D interfered with the transcriptional activation promoted by the cooperative interaction of MEF2A and GEF. A transcriptional repressor form of MEF2A that is sumoylated at lys403 promoted dendritic claw differentiation.

Anti-MEF2A Antibody Images

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Anti-MEF2A Antibody
Anti-MEF2A antibody, PA1823, Western blotting
All lanes: Anti MEF2A (PA1823) at 0.5ug/ml
Lane 1: Rat Heart Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HT1080Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mef2 antibody, mef2a antibody