Data & Images
|Product Name||Anti-MEF2C Antibody|
|Description||Rabbit IgG polyclonal antibody for Myocyte-specific enhancer factor 2C(MEF2C) detection. Tested with WB, IHC-P in Human;Rat.|
|Cite This Product||Anti-MEF2C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1824)|
|Replacement Item||This antibody may replace the following items: sc-130201|sc-13266|sc-13266-X|sc-13268|sc-13268-X|sc-13917|sc-13917-X|sc-13919-R|sc-13919-X|sc-13920-R|sc-13920-X|sc-17785|sc-17785-X|sc-313|sc-313-X|sc-365862|sc-365862-X|sc-377535|sc-377535-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2C(406-419aa SRYPQHTRHEAGRS), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Myocyte-specific enhancer factor 2C|
|Molecular Weight||51221 MW|
|Protein Function||Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle- specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2. .|
|Tissue Specificity||Expressed in brain and skeletal muscle. .|
|Sequence Similarities||Belongs to the MEF2 family.|
|Alternative Names||Myocyte-specific enhancer factor 2C;MEF2C;|
|Research Areas|||signal transduction|signaling pathway|calcium signaling|calmodulin pathway| neuroscience|neurology process|neurogenesis| epigenetics and nuclear signaling|transcription|other factors|domain families|developmental families|transcription factors| stem cells|mesenchymal stem cells|myogenesis| cardiovascular|heart|cardiogenesis|transcription factors/regulators|hypertrophy||
Background for Myocyte-specific enhancer factor 2C
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MEF2C Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MEF2C Antibody Images
Click the images to enlarge.
IHC(P): Human Intestine Tissue
Lane 1: Rat Testis Tissue Lysate
Lane 2: COLO320 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,