Product Info Summary
SKU: | M02916 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MEK3/MEK6 MAP2K3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02916
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MEK3/MEK6 MAP2K3 Rabbit Monoclonal Antibody catalog # M02916. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK3/MEK6 MAP2K3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02916)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HDA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MEK3/MEK6
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M02916 is reactive to MAP2K3 in Human, Mouse, Rat
Applications
M02916 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
150 kDa
Calculated molecular weight
39.318kDa
Background of MKK3/MEK3
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:100-1:500
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Western blot analysis of MEK3/MEK6 expression in HeLa cell lysate.
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded mouse spleen, using MEK3/MEK6 Antibody.
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Immunofluorescent analysis of Hela cells, using MEK3/MEK6 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K3 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K3
Full Name
Dual specificity mitogen-activated protein kinase kinase 3
Weight
39.318kDa
Superfamily
protein kinase superfamily
Alternative Names
MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3PRKMK3dual specificity mitogen-activated protein kinase kinase 3; MEK3; MEK3MEK 3; mitogen activated protein kinase kinase 3; mitogen-activated protein kinase kinase 3; MKK3; MKK3EC 2.7.12.2; PRKMK3 MAP2K3 MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2 mitogen-activated protein kinase kinase 3 dual specificity mitogen-activated protein kinase kinase 3|MAP kinase kinase 3|MAPK/ERK kinase 3|MAPKK 3|MEK 3|SAPK kinase 2|stress-activated protein kinase kinase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K3, check out the MAP2K3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK3/MEK6 MAP2K3 Rabbit Monoclonal Antibody (M02916)
Hello CJ!
M02916 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu G, Zhang Y, Wei J, Jia W, Ge Z, Zhang Z, Liu X. Bmc Cancer. 2013 Oct 10;13:469. Doi: 10.1186/1471-2407-13-469. Microrna-21 Promotes Hepatocellular Carcinoma Hepg2 Cell Proliferation Through Repression Of Mitogen-Activated Protein Kinase-Kinase 3.
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