Show Order Info
SKU:PA1408
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-MGMT Antibody
SKU/Catalog Number PA1408
Description Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase(MGMT) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-MGMT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1408)
Replacement Item This antibody may replace the following items: sc-56157|sc-28241|sc-166491|sc-166528|sc-166527|sc-8827|sc-8826|sc-56432|sc-8824|sc-33674|sc-271155|sc-271154|sc-8825|sc-514277 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human MGMT(87-101aa PVFQQESFTRQVLWK), different from the related rat and mouse sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MGMT
Protein Name Methylated-DNA--protein-cysteine methyltransferase
Molecular Weight 21646 MW
Protein Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Subcellular Localization Nucleus.
Uniprot ID P16455
Alternative Names Methylated-DNA--protein-cysteine methyltransferase;2.1.1.63;6-O-methylguanine-DNA methyltransferase;MGMT;O-6-methylguanine-DNA-alkyltransferase;MGMT;
Research Areas |epigenetics and nuclear signaling|dna methylation|dnmts| epigenetics and nuclear signaling|dna / rna|dna damage & repair|direct chemical reversal|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Methylated-DNA--protein-cysteine methyltransferase

Methylated-DNA-protein-cysteine methytransferase(MGMT) is an enzyme that in humans is encoded by the MGMT gene. O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. MGMT gene is mapped to chromosome 10. Rydberg et al.(1990) concluded that the methyltransferase gene spans at least 15 kb. Gardner et al.(1991) refined the localization of the gene to 10q24.33-qter. Methylation of the gene's promoter plays a significant role in carcinogenesis.

Anti-MGMT Antibody Images

Click the images to enlarge.

Anti-MGMT Antibody
Anti-MGMT antibody, PA1408, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: Rat Thymus Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: JURKAT Cell Lysate
Lane 8: RAJI Cell Lysate
Write a review for PA1408

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.