Anti-MIA Antibody

MIA antibody

Boster Bio Anti-MIA Antibody (Catalog# A01570-2). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01570-2
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, IF (paraffin)

Product Name

Anti-MIA Antibody

View all MIA Antibodies

SKU/Catalog Number

A01570-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MIA Antibody (Catalog# A01570-2). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MIA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01570-2)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human MIA. Immunogen sequence location: 82-131

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01570-2 is reactive to MIA in Human, Mouse, Rat

Applications

A01570-2 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

14.509kDa

Background of MIA

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For MIA (Source: Uniprot.org, NCBI)

Gene Name

MIA

Full Name

Melanoma-derived growth regulatory protein

Weight

14.509kDa

Superfamily

MIA/OTOR family

Alternative Names

CD-RAP; Melanoma inhibitory activity protein; melanoma inhibitory activity; melanoma-derived growth regulatory protein; MIA MIA CD-RAP MIA SH3 domain containing melanoma-derived growth regulatory protein|melanoma inhibitory activity

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MIA, check out the MIA Infographic

MIA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MIA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-MIA Antibody

Question

I have attached the WB image, lot number and protocol we used for skin using anti-MIA antibody A01570-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-26

Question

Will anti-MIA antibody A01570-2 work for IHC with skin?

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

According to the expression profile of skin, MIA is highly expressed in skin. So, it is likely that anti-MIA antibody A01570-2 will work for IHC with skin.

Boster Scientific Support

Answered: 2019-05-30

Question

We are currently using anti-MIA antibody A01570-2 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

V. Li

Verified customer

Asked: 2014-12-22

Answer

The anti-MIA antibody (A01570-2) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-12-22

Order DetailsPrice
A01570-2

100 μL

$335

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