Anti-Mib1/Mindbomb Antibody Picoband™

Mind Bomb 1/MIB1 antibody

Boster Bio Anti-Mib1/Mindbomb Antibody Picoband™ catalog # A01387-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01387-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Customers Who Bought This Also Bought

Product Name

Anti-Mib1/Mindbomb Antibody Picoband™

View all Mind Bomb 1/MIB1 Antibodies

SKU/Catalog Number

A01387-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mib1/Mindbomb Antibody Picoband™ catalog # A01387-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mib1/Mindbomb Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01387-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Mib1 Mindbomb recombinant protein (Position: H465-E732).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01387-1 is reactive to MIB1 in Human, Mouse, Rat

Applications

A01387-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

110.136kDa

Background of Mind Bomb 1/MIB1

E3 ubiquitin-protein ligase MIB1 is an enzyme that in humans is encoded by the MIB1 gene. It is mapped to 18q11.2. This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For MIB1 (Source: Uniprot.org, NCBI)

Gene Name

MIB1

Full Name

E3 ubiquitin-protein ligase MIB1

Weight

110.136kDa

Alternative Names

DAPK-interacting protein 1; DIP1; DIP-1FLJ90676; DKFZp686I0769; DKFZp761M1710; E3 ubiquitin-protein ligase MIB1; EC 6.3.2; EC 6.3.2.-; KIAA1323; MGC129660; MIB; MIB1; Mind Bomb 1; Mind bomb homolog 1; mindbomb homolog 1 (Drosophila); ubiquitin ligase mind bomb; ubiquitin ligase protein MIB1; Zinc finger ZZ type with ankyrin repeat domain protein 2; ZZANK2; ZZANK2MGC129659; ZZZ6 MIB1 DIP-1, DIP1, LVNC7, MIB, ZZANK2, ZZZ6 MIB E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase MIB1|DAPK-interacting protein 1|RING-type E3 ubiquitin transferase MIB1|mindbomb E3 ubiquitin protein ligase 1|ubiquitin ligase mind bomb|zinc finger ZZ type with ankyrin repeat domain protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MIB1, check out the MIB1 Infographic

MIB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MIB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A01387-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Mib1/Mindbomb Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Mib1/Mindbomb Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-Mib1/Mindbomb Antibody Picoband™

Question

We ordered your anti-Mib1/Mindbomb antibody for ELISA on brain in the past. I am using human, and We intend to use the antibody for WB next. We are interested in examining brain as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for WB?

K. Evans

Verified customer

Asked: 2020-04-09

Answer

I took a look at the website and datasheets of our anti-Mib1/Mindbomb antibody and it seems that A01387-1 has been validated on human in both ELISA and WB. Thus A01387-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-09

Question

My team were well pleased with the WB result of your anti-Mib1/Mindbomb antibody. However we have seen positive staining in lung cytoplasm. using this antibody. Is that expected? Could you tell me where is MIB1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

From literature, lung does express MIB1. Generally MIB1 expresses in cytoplasm. Regarding which tissues have MIB1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Endometrial tumor, Pubmed ID: 17974005
Brain, Pubmed ID: 10718198
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-03-19

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Mib1/Mindbomb antibody A01387-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-13

Question

I would like using your anti-Mib1/Mindbomb antibody for constitutive signaling by notch1 hd domain mutants studies. Has this antibody been tested with western blotting on human a375 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

Thank you for your inquiry. This A01387-1 anti-Mib1/Mindbomb antibody is validated on human a375 whole cell lysate, hepg2 whole cell lysate. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-09

Question

We are currently using anti-Mib1/Mindbomb antibody A01387-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

The anti-Mib1/Mindbomb antibody (A01387-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-19

Question

We have seen staining in rat brain. Any tips? Is anti-Mib1/Mindbomb antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

From literature brain does express MIB1. From Uniprot.org, MIB1 is expressed in kidney, lung, amygdala endometrial tumor, brain, placenta, liver, among other tissues. Regarding which tissues have MIB1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Endometrial tumor, Pubmed ID: 17974005
Brain, Pubmed ID: 10718198
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Placenta, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-11-05

Question

My lab would like to test anti-Mib1/Mindbomb antibody A01387-1 on mouse liver for research purposes, then I may be interested in using anti-Mib1/Mindbomb antibody A01387-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

The products we sell, including anti-Mib1/Mindbomb antibody A01387-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-01

Question

Is this A01387-1 anti-Mib1/Mindbomb antibody reactive to the isotypes of MIB1?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

The immunogen of A01387-1 anti-Mib1/Mindbomb antibody is E. coli-derived human Mib1 Mindbomb recombinant protein (Position: H465-E732). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-17

Question

Is a blocking peptide available for product anti-Mib1/Mindbomb antibody (A01387-1)?

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

We do provide the blocking peptide for product anti-Mib1/Mindbomb antibody (A01387-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-14

Question

Please see the WB image, lot number and protocol we used for liver using anti-Mib1/Mindbomb antibody A01387-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-12

Question

I was wanting to use your anti-Mib1/Mindbomb antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?

Verified Customer

Verified customer

Asked: 2019-07-29

Answer

You can see on the product datasheet, A01387-1 anti-Mib1/Mindbomb antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-29

Question

Does A01387-1 anti-Mib1/Mindbomb antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

It shows on the product datasheet, A01387-1 anti-Mib1/Mindbomb antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-25

Question

My question regarding product A01387-1, anti-Mib1/Mindbomb antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01387-1 anti-Mib1/Mindbomb antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-27

Question

I see that the anti-Mib1/Mindbomb antibody A01387-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-12-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-12-28

Question

Do you have a BSA free version of anti-Mib1/Mindbomb antibody A01387-1 available?

C. Roberts

Verified customer

Asked: 2015-07-20

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Mib1/Mindbomb antibody A01387-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-07-20

Question

Would anti-Mib1/Mindbomb antibody A01387-1 work for WB with liver?

C. Moore

Verified customer

Asked: 2013-02-06

Answer

According to the expression profile of liver, MIB1 is highly expressed in liver. So, it is likely that anti-Mib1/Mindbomb antibody A01387-1 will work for WB with liver.

Boster Scientific Support

Answered: 2013-02-06

Order DetailsPrice
A01387-1

100μg

$370
A01387-1-10ug

10μg sample (liquid)

$99
A01387-1-Biotin

100 μg Biotin conjugated

$570
A01387-1-Cy3

100 μg Cy3 conjugated

$570
A01387-1-Dylight488

100 μg Dylight488 conjugated

$570
A01387-1-Dylight550

100 μg Dylight550 conjugated

$570
A01387-1-Dylight594

100 μg Dylight594 conjugated

$570
A01387-1-FITC

100 μg FITC conjugated

$570
A01387-1-HRP

100 μg HRP conjugated

$570
A01387-1-APC

100 μg APC conjugated

$670
A01387-1-PE

100 μg PE conjugated

$670
A01387-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A01387-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.