Anti-MIP-3 Alpha/CCL20 Antibody Picoband™

CCL20/MIP-3 alpha antibody

Boster Bio Anti-MIP-3 Alpha/CCL20 Antibody Picoband™ catalog # A00748-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A00748-2
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-MIP-3 Alpha/CCL20 Antibody Picoband™

View all CCL20/MIP-3 alpha Antibodies

SKU/Catalog Number

A00748-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MIP-3 Alpha/CCL20 Antibody Picoband™ catalog # A00748-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MIP-3 Alpha/CCL20 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00748-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse MIP-3 Alpha/CCL20 recombinant protein (Position: A28-M97).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00748-2 is reactive to CCL20 in Mouse, Rat

Applications

A00748-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

14 kDa

Calculated molecular weight

10.762kDa

Background of CCL20/MIP-3 alpha

Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CCL20 (Source: Uniprot.org, NCBI)

Gene Name

CCL20

Full Name

C-C motif chemokine 20

Weight

10.762kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38 CCL20 CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38 C-C motif chemokine ligand 20 C-C motif chemokine 20|CC chemokine LARC|beta chemokine exodus-1|chemokine (C-C motif) ligand 20|liver and activation-regulated chemokine|macrophage inflammatory protein 3 alpha|small inducible cytokine subfamily A (Cys-Cys), member 20|small-inducible cytokine A20

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCL20, check out the CCL20 Infographic

CCL20 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCL20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-MIP-3 Alpha/CCL20 Antibody Picoband™

Question

Can you help my question with product A00748-2, anti-MIP-3 Alpha/CCL20 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00748-2 anti-MIP-3 Alpha/CCL20 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-05-06

Question

We are currently using anti-MIP-3 Alpha/CCL20 antibody A00748-2 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

The anti-MIP-3 Alpha/CCL20 antibody (A00748-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-10

Question

Would anti-MIP-3 Alpha/CCL20 antibody A00748-2 work for ELISA with pancreas?

Verified Customer

Verified customer

Asked: 2018-09-19

Answer

According to the expression profile of pancreas, CCL20 is highly expressed in pancreas. So, it is likely that anti-MIP-3 Alpha/CCL20 antibody A00748-2 will work for ELISA with pancreas.

Boster Scientific Support

Answered: 2018-09-19

Question

Is a blocking peptide available for product anti-MIP-3 Alpha/CCL20 antibody (A00748-2)?

J. Patel

Verified customer

Asked: 2013-06-13

Answer

We do provide the blocking peptide for product anti-MIP-3 Alpha/CCL20 antibody (A00748-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-06-13

Order DetailsPrice
A00748-2

100μg

$370
A00748-2-10ug

10μg sample (liquid)

$99
A00748-2-Biotin

100 μg Biotin conjugated

$570
A00748-2-Cy3

100 μg Cy3 conjugated

$570
A00748-2-Dylight488

100 μg Dylight488 conjugated

$570
A00748-2-Dylight550

100 μg Dylight550 conjugated

$570
A00748-2-Dylight594

100 μg Dylight594 conjugated

$570
A00748-2-FITC

100 μg FITC conjugated

$570
A00748-2-HRP

100 μg HRP conjugated

$570
A00748-2-APC

100 μg APC conjugated

$670
A00748-2-PE

100 μg PE conjugated

$670
A00748-2-carrier-free

Carrier Free

$370

More conjugates/formats

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A00748-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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