Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody

Pyruvate Dehydrogenase Kinase 1/PDK1 antibody

Boster Bio Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody catalog # PA1993. Tested in IHC, WB applications. This antibody reacts with Human, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1993
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody

View all Pyruvate Dehydrogenase Kinase 1/PDK1 Antibodies

SKU/Catalog Number

PA1993

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody catalog # PA1993. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1993)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1993 is reactive to PDK1 in Human, Rat

Applications

PA1993 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

85-91 kDa

Calculated molecular weight

49.244kDa

Background of Pyruvate Dehydrogenase Kinase 1/PDK1

PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For PDK1 (Source: Uniprot.org, NCBI)

Gene Name

PDK1

Full Name

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

Weight

49.244kDa

Superfamily

PDK/BCKDK protein kinase family

Alternative Names

EC 2.7.11; EC 2.7.11.2; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; PDH kinase 1; PDK1; PDK-1; PKB kinase; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1; Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial PDK1 pyruvate dehydrogenase kinase 1 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial|pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial|PDH kinase 1|mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1|pyruvate dehydrogenase kinase, isoenzyme 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDK1, check out the PDK1 Infographic

PDK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1993 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPAR? pathway in hepatocellular carcinoma

Xu K, Ding Q, Fang Z, Zheng J, Gao P, Lu Y, Zhang Y. Cancer Gene Ther. 2010 Mar;17(3):212-22. Doi: 10.1038/Cgt.2009.66. Epub 2009 Oct 9. Silencing Of Hif-1Alpha Suppresses Tumorigenicity Of Renal Cell Carcinoma Through Induction Of Apoptosis.

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6 Customer Q&As for Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody

Question

I have a question about product PA1993, anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1993 anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-07

Question

I was wanting to use your anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

N. Parker

Verified customer

Asked: 2019-10-01

Answer

As indicated on the product datasheet, PA1993 anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-01

Question

I would like to test anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody PA1993 on human liver for research purposes, then I may be interested in using anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody PA1993 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

The products we sell, including anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody PA1993, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-09

Question

Is a blocking peptide available for product anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody (PA1993)?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

We do provide the blocking peptide for product anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody (PA1993). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-02

Question

We are currently using anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody PA1993 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on bovine tissues as well?

B. Jha

Verified customer

Asked: 2016-08-01

Answer

The anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody (PA1993) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-01

Question

Is this PA1993 anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody reactive to the isotypes of PDK1?

A. Kulkarni

Verified customer

Asked: 2016-04-15

Answer

The immunogen of PA1993 anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1(408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-04-15

Order DetailsPrice
PA1993

100μg

$370
PA1993-10ug

10μg sample (liquid)

$99
PA1993-Biotin

100 μg Biotin conjugated

$570
PA1993-Cy3

100 μg Cy3 conjugated

$570
PA1993-Dylight488

100 μg Dylight488 conjugated

$570
PA1993-Dylight550

100 μg Dylight550 conjugated

$570
PA1993-Dylight594

100 μg Dylight594 conjugated

$570
PA1993-FITC

100 μg FITC conjugated

$570
PA1993-HRP

100 μg HRP conjugated

$570
PA1993-APC

100 μg APC conjugated

$670
PA1993-PE

100 μg PE conjugated

$670
PA1993-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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PA1993
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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