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SKU:PA1993
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Mitochondrial Pyruvate Dehydrogenase Kinase 1 Antibody
Description Rabbit IgG polyclonal antibody for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial(PDK1) detection. Tested with WB, IHC-P in Human;Rat.
Cite This Product Anti-Mitochondrial Pyruvate Dehydrogenase Kinase 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1993)
Replacement Item This antibody may replace the following items: sc-30691|sc-7140|sc-30692 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1(408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name PDK1
Protein Name [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
Molecular Weight 49244 MW
Protein Function Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. .
Tissue Specificity Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. .
Sequence Similarities Belongs to the PDK/BCKDK protein kinase family.
Subcellular Localization Mitochondrion matrix .
Uniprot ID Q15118
Alternative Names [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial;2.7.11.2;Pyruvate dehydrogenase kinase isoform 1;PDH kinase 1;PDK1;PDHK1;
Research Areas |signal transduction|metabolism|energy metabolism| signal transduction|mitochondrial| epigenetics and nuclear signaling|chromatin modifying enzymes|methylation|lysine methylation| cancer|oncoproteins/suppressors|tumor suppressors|cancer metabolism|metabolic signaling pathway|metabolism of carbohydrates|integration of energy metabolism|cellular metabolic process| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|metabolic signaling pathways|carbohydrate metabolism|energy transfer pathways|integration of energy|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

PDK1(Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Anti-Mitochondrial Pyruvate Dehydrogenase Kinase 1 Antibody Images

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Anti-Mitochondrial Pyruvate Dehydrogenase Kinase 1 Antibody
Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, PA1993, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: M231 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: SW620 Cell Lysate
Anti-Mitochondrial Pyruvate Dehydrogenase Kinase 1 Antibody
Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, PA1993, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
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Publications

Xu K, Ding Q, Fang Z, Zheng J, Gao P, Lu Y, Zhang Y. Cancer Gene Ther. 2010 Mar;17(3):212-22. Doi: 10.1038/Cgt.2009.66. Epub 2009 Oct 9. Silencing Of Hif-1Alpha Suppresses Tumorigenicity Of Renal Cell Carcinoma Through Induction Of Apoptosis.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is pdk1 antibody