Data & Images
|Product Name||Anti-Mitofusin 2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Mitofusin-2(MFN2) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-Mitofusin 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1051)|
|Replacement Item||This antibody may replace the following items: sc-100560|sc-30366|sc-50331|sc-30367 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Mitofusin 2(6-24aa SRCNSIVTVKKNKRHMAEV), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||86402 MW|
|Protein Function||Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. .|
|Tissue Specificity||Ubiquitous; expressed at low level. Highly expressed in heart and kidney. .|
|Sequence Similarities||Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.|
|Subcellular Localization||Mitochondrion outer membrane ; Multi- pass membrane protein . Colocalizes with BAX during apoptosis.|
|Alternative Names||Mitofusin-2;3.6.5.-;Transmembrane GTPase MFN2;MFN2;CPRP1, KIAA0214;|
|Research Areas|||tags & cell markers|subcellular markers|organelles|mitochondria| cell biology|apoptosis|mitochondrial| signal transduction|metabolism| neuroscience|neurology process|neurodegenerative disease| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|mitophagy fission and fusion|metabolism processes|types of disease|cancer| cancer|cell death||
Background for Mitofusin-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Mitofusin 2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Mitofusin 2 Antibody Images
Click the images to enlarge.
All lanes: Anti Mitofusin 2 (PA1051) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 86KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,