Anti-MLKL Rabbit Monoclonal Antibody

Mlkl antibody

Boster Bio Anti-MLKL Rabbit Monoclonal Antibody catalog # M00535. Tested in WB, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M00535
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-MLKL Rabbit Monoclonal Antibody

View all Mlkl Antibodies

SKU/Catalog Number

M00535

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-MLKL Rabbit Monoclonal Antibody catalog # M00535. Tested in WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MLKL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00535)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DGB-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human MLKL

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00535 is reactive to Mlkl in Human

Applications

M00535 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

54.317kDa

Background of Mlkl

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 50-200

Validation Images & Assay Conditions

Gene/Protein Information For Mlkl (Source: Uniprot.org, NCBI)

Gene Name

Mlkl

Full Name

Mixed lineage kinase domain-like protein

Weight

54.317kDa

Superfamily

protein kinase superfamily

Alternative Names

FLJ34389; mixed lineage kinase domain-like protein; mixed lineage kinase domain-like; MLKL Mlkl|9130019I15Rik|mixed lineage kinase domain-like|mixed lineage kinase domain-like protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Mlkl, check out the Mlkl Infographic

Mlkl infographic

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In this infographic, you will see the following information for Mlkl: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-MLKL Rabbit Monoclonal Antibody

Question

See attached the WB image, lot number and protocol we used for chondrocyte {eco:0000312 using anti-MLKL Rabbit Monoclonal antibody M00535. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

Is a blocking peptide available for product anti-MLKL Rabbit Monoclonal antibody (M00535)?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

We do provide the blocking peptide for product anti-MLKL Rabbit Monoclonal antibody (M00535). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-16

Question

My question regarding product M00535, anti-MLKL Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00535 anti-MLKL Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-28

Question

I am interested in to test anti-MLKL Rabbit Monoclonal antibody M00535 on human chondrocyte {eco:0000312 for research purposes, then I may be interested in using anti-MLKL Rabbit Monoclonal antibody M00535 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Zhang

Verified customer

Asked: 2018-12-10

Answer

The products we sell, including anti-MLKL Rabbit Monoclonal antibody M00535, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-10

Question

Is this M00535 anti-MLKL Rabbit Monoclonal antibody reactive to the isotypes of MLKL?

E. Singh

Verified customer

Asked: 2018-01-17

Answer

The immunogen of M00535 anti-MLKL Rabbit Monoclonal antibody is A synthesized peptide derived from human MLKL. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-01-17

Order DetailsPrice
M00535

100μL

$370
M00535-30ul

30μL sample

$99
M00535-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M00535
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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