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SKU:PA1357
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-MMP-9 Antibody
Description Rabbit IgG polyclonal antibody for Matrix metalloproteinase-9(MMP9) detection. Tested with WB in Human.
Cite This Product Anti-MMP-9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1357)
Replacement Item This antibody may replace the following items: sc-87630|sc-87629 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human MMP-9(689-705aa NQVDQVGYVTYDILQCP), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MMP9
Protein Name Matrix metalloproteinase-9(MMP-9)
Molecular Weight 78458 MW
Protein Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-

-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. .
Tissue Specificity Produced by normal alveolar macrophages and granulocytes.
Sequence Similarities Belongs to the peptidase M10A family.
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Uniprot ID P14780
Alternative Names Matrix metalloproteinase-9;MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;67 kDa matrix metalloproteinase-9;82 kDa matrix metalloproteinase-9;MMP9;CLG4B;
Research Areas |cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|mmp| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes| cancer|invasion/microenvironment|mmps| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|tumor biomarkers| cardiovascular|atherosclerosis|thrombosis| kits/ lysates/ other|elisa kits|atherosclerotic proteins elisa kits|cancer proteins elisa kits|protease inhibitors elisa kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Matrix metalloproteinase-9(MMP-9)

Matrix metallopeptidase 9(MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B(GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase(MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Anti-MMP-9 Antibody Images

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Anti-MMP-9 Antibody
Anti-MMP-9 antibody, PA1357, Western blotting
Lane 1: Rat Embryo Tissue Lysate
Lane 2: MM453 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: JURKAT Cell Lysate
Lane 6: HT1080 Cell Lysate
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Publications

Sun Y, Liu M, Yang B, Lu J, Li B. Cancer Invest. 2008 Dec;26(10):984-9. Doi: 10.1080/07357900802072897. Inhibition Of Laryngeal Cancer Cell Invasion And Growth With Lentiviral-Vector Delivered Short Hairpin Rna Targeting Human Mmp-9 Gene.
Meng L, Yu B. Int J Mol Med. 2011 Nov;28(5):777-83. Doi: 10.3892/Ijmm.2011.744. Epub 2011 Jul 8. Oxygen- And Glucose-Deprived Culture Promotes Cell Proliferation And Invasion Of Vascular Smooth Muscle Cells.
Wu G, Wang L, Hong Z, Li C, Long X, Sheng F. Neurol Res. 2011 Apr;33(3):300-6. Doi: 10.1179/016164110X12759951866993. Epub 2010 Aug 16. Effects Of Minimally Invasive Procedures For Removal Of Intracranial Hematoma On Matrix Metalloproteinase Expre...
Cheng Ys, Dai Dz, Dai Y. Acta Pharmacol Sin. 2009 Aug;30(8):1099-106. Doi: 10.1038/Aps.2009.104. Epub 2009 Jul 13. Isoproterenol Disperses Distribution Of Nadph Oxidase, Mmp-9, And Ppkcepsilon In The Heart, Which Are Mitigated By Endothelin Recept...
Chen Y, Chen Y, Huang L, Yu J. J Dermatol. 2012 Apr;39(4):339-43. Doi: 10.1111/J.1346-8138.2011.01441.X. Epub 2011 Dec 13. Evaluation Of Heparanase And Matrix Metalloproteinase-9 In Patients With Cutaneous Malignant Melanoma.
Rao Ym, Ji M, Chen Ch, Shi Hr. J Huazhong Univ Sci Technolog Med Sci. 2013 Apr;33(2):266-71. Doi: 10.1007/S11596-013-1109-8. Epub 2013 Apr 17. Effect Of Sirna Targeting Mta1 On Metastasis Malignant Phenotype Of Ovarian Cancer A2780 Cells.
Pang B, Fan H, Zhang Iy, Liu B, Feng B, Meng L, Zhang R, Sadeghi S, Guo H, Pang Q. J Neurooncol. 2012 Feb;106(3):543-9. Doi: 10.1007/S11060-011-0710-6. Epub 2011 Oct 8. Hmga1 Expression In Human Gliomas And Its Correlation With Tumor Proliferation...
Yang Tq, Lu Xj, Wu Tf, Ding Dd, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Guo Lc, Zhang Y, Huang Yl, Zhou Yx, Du Zw. Cancer Sci. 2014 Mar;105(3):265-71. Doi: 10.1111/Cas.12351. Epub 2014 Feb 11. Microrna-16 Inhibits Glioma Cell Growth And Invasion T...
Li Xt, Wang Hz, Wu Zw, Yang Tq, Zhao Zh, Chen Gl, Xie Xs, Li B, Wei Yx, Huang Yl, Zhou Yx, Du Zw. Cell Mol Neurobiol. 2015 Jul;35(5):679-87. Doi: 10.1007/S10571-015-0163-0. Epub 2015 Feb 8. Mir-494-3P Regulates Cellular Proliferation, Invasion, Mi...
Song Y, Zou H, Wang G, Yang H, Xie Z, Bi J. Neural Regen Res. 2012 Jun 15;7(17):1325-30. Doi: 10.3969/J.Issn.1673-5374.2012.17.007. Matrix Metalloproteinase-9 And Tissue Inhibitor Of Metalloproteinase-1 Expression In Early Focal Cerebral Infarctio...
Zhao Q, Xue Y, Liu Zd, Li H, Wang Jf, Li Zj, Wang Ym, Dong P, Xue Ch. J Food Sci. 2010 Nov-Dec;75(9):H280-8. Doi: 10.1111/J.1750-3841.2010.01837.X. Epub 2010 Oct 15. Differential Effects Of Sulfated Triterpene Glycosides, Holothurin A1, And 24-Deh...
Liu Ww, Zeng Zy, Wu Ql, Hou Jh, Chen Yy. Otolaryngol Head Neck Surg. 2005 Mar;132(3):395-400. Overexpression Of Mmp-2 In Laryngeal Squamous Cell Carcinoma: A Potential Indicator For Poor Prognosis.
Liu T, Zheng X, Chen J, Wang N, Xiao J, Zhang D, Yin Z, Li W, Chen S. J Huazhong Univ Sci Technolog Med Sci. 2011 Dec;31(6):819-23. Doi: 10.1007/S11596-011-0683-X. Epub 2011 Dec 16. Effect Of Human Cytomegalovirus On Invasive Capability Of Early P...
Fang R, Zhang G, Guo Q, Ning F, Wang H, Cai S, Du J. Cancer Lett. 2013 Jun 1;333(1):66-75. Doi: 10.1016/J.Canlet.2013.01.014. Epub 2013 Jan 22. Nodal Promotes Aggressive Phenotype Via Snail-Mediated Epithelial-Mesenchymal Transition In Murine Mela...
Shi Ch, Wang Ws, Zhang Zd, Li Cj, Guo Fj, Li F, Chen Am. J Huazhong Univ Sci Technolog Med Sci. 2015 Feb;35(1):111-6. Doi: 10.1007/S11596-015-1398-1. Epub 2015 Feb 12. Effect Of Lentivirus-Mediated Upa Silencing On The Proliferation And Apoptosis ...
Zhao Y, Zong Zh, Xu Hm. Gynecol Oncol. 2010 Mar;116(3):563-71. Doi: 10.1016/J.Ygyno.2009.11.015. Rhoc Expression Level Is Correlated With The Clinicopathological Characteristics Of Ovarian Cancer And The Expression Levels Of Rock-I, Vegf, And Mmp9.
Liu Y, Zhou Y, Zhang Xs, Shen Bz. Oncol Lett. 2011 Nov;2(6):1171-1175. Epub 2011 Aug 17. Expression Of Vegf And Mmp-9 And Mri Imaging Changes In Cerebral Glioma.
Zhang Lh, Jia Yl, Lin Xx, Zhang Hq, Dong Xw, Zhao Jm, Shen J, Shen Hj, Li Ff, Yan Xf, Li W, Zhao Yq, Xie Qm. Biochim Biophys Acta. 2013 Aug;1830(8):4148-59. Doi: 10.1016/J.Bbagen.2013.04.008. Epub 2013 Apr 11. Ad-1, A Novel Ginsenoside Derivative,...
Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...
Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.
Cen J, Liu L, Li Ms, He L, Wang Lj, Liu Yq, Liu M, Ji Bs. J Pharm Pharmacol. 2013 May;65(5):665-72. Doi: 10.1111/Jphp.12033. Epub 2013 Feb 26. Alteration In P-Glycoprotein At The Blood-Brain Barrier In The Early Period Of Mcao In Rats.
Hu P, Wang J, Zhao Xq, Hu B, Lu L, Qin Yh. Mol Biol Rep. 2013 Feb;40(2):1429-41. Doi: 10.1007/S11033-012-2186-7. Epub 2012 Oct 17. Overexpressed C-Type Natriuretic Peptide Serves As An Early Compensatory Response To Counteract Extracellular Matrix...
Jin F, Jiang S, Yang D, Zhang X, Yang Y, Zhang Y, Li K, Yang Y, Ma S. Biochem Biophys Res Commun. 2012 Nov 9;428(1):86-92. Doi: 10.1016/J.Bbrc.2012.10.011. Epub 2012 Oct 8. Acipimox Attenuates Atherosclerosis And Enhances Plaque Stability In Apoe-...
Jiang S, Jin F, Li D, Zhang X, Yang Y, Yang D, Li K, Yang Y, Ma S. High Alt Med Biol. 2013 Jun;14(2):175-80. Doi: 10.1089/Ham.2012.1083. Intermittent Hypobaric Hypoxia Promotes Atherosclerotic Plaque Instability In Apoe-Deficient Mice.
Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.
Wu L, Zhang W, Tang Yh, Li H, Chen By, Zhang Gm, Deng Cq. Phytomedicine. 2010 Mar;17(3-4):233-40. Doi: 10.1016/J.Phymed.2009.07.021. Epub 2009 Sep 11. Effect Of Total Saponins Of "Panax Notoginseng Root" On Aortic Intimal Hyperplasia And The Expre...
Zhou Y, Tan X, Kuang W, Liu L, Wan L. Transl Res. 2012 Jun;159(6):464-72. Doi: 10.1016/J.Trsl.2011.09.007. Epub 2011 Oct 24. Erythromycin Ameliorates Cigarette-Smoke-Induced Emphysema And Inflammation In Rats.
Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.
Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z. Virol J. 2011 Mar 11;8:114. Doi: 10.1186/1743-422X-8-114. In Vitro Study On Human Cytomegalovirus Affecting Early Pregnancy Villous Evt'S Invasion Function.
Jiang Z, Han B, Li H, Li X, Yang Y, Liu W. Carbohydr Polym. 2015 Jul 10;125:53-60. Doi: 10.1016/J.Carbpol.2015.02.039. Epub 2015 Feb 26. Preparation And Anti-Tumor Metastasis Of Carboxymethyl Chitosan.
Chen Sz, Yao Hq, Zhu Sz, Li Qy, Guo Gh, Yu J. Oncol Lett. 2015 Feb;9(2):915-919. Epub 2014 Dec 4. Expression Levels Of Matrix Metalloproteinase-9 In Human Gastric Carcinoma.
Huang Z, Yang Y, Jiang Y, Shao J, Sun X, Chen J, Dong L, Zhang J. Biomaterials. 2013 Jan;34(3):746-55. Doi: 10.1016/J.Biomaterials.2012.09.062. Epub 2012 Oct 27. Anti-Tumor Immune Responses Of Tumor-Associated Macrophages Via Toll-Like Receptor 4 ...
Zhou Ch, Wan Yy, Chu Xh, Song Z, Xing Sh, Wu Yq, Yin Xx. Oncol Lett. 2012 Dec;4(6):1259-1263. Epub 2012 Sep 21. Urotensin Ii Contributes To The Formation Of Lung Adenocarcinoma Inflammatory Microenvironment Through The Nf-??b Pathway In Tumor-Beari...
Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mmp 9 antibody, mmp-9 antibody, mmp9 antibody