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Pack Size:100μg/vial
Validated Species:Mouse, Rat
Application:IHC, WB
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Product Name Anti-MMP-7 Antibody
SKU/Catalog Number PA1471
Description Rabbit IgG polyclonal antibody for Matrilysin(MMP7) detection. Tested with WB, IHC-P in Mouse;Rat.
Cite This Product Anti-MMP-7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1471)
Replacement Item This antibody may replace the following items: sc-101566|sc-26674|sc-30071|sc-58388|sc-80205|sc-8832 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse MMP7(236-252aa YQRDYSEDFSLTKDDIA), different from the related rat sequence by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Mmp7
Protein Name Matrilysin
Molecular Weight 29755 MW
Protein Function Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase (By similarity). .
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Uniprot ID Q10738
Alternative Names Matrilysin;;Matrin;Matrix metalloproteinase-7;MMP-7;Pump-1 protease;Uterine metalloproteinase;Mmp7;
Research Areas |cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|mmp| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes| cancer|invasion/microenvironment|mmps| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Matrilysin

MMP7, matrix metalloproteinase-7, is an enzyme that in humans is encoded by the MMP7 gene. The MMP7 protein has 267 amino acids and is significantly shorter than stromelysin or collagenase. By Southern and PCR analysis of 2 different panels of somatic cell hybrids and by isotopic in situ hybridization, the MMP7 gene is mapped to 11q21-q22. It is a member of matrix metalloproteinase family. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa.

Anti-MMP-7 Antibody Images

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Anti-MMP-7 Antibody
Anti-MMP7 antibody, PA1471, Western blotting
Lane 1: Recombinant Mouse MMP-7 Protein 10ng
Lane 2: Recombinant Mouse MMP-7 Protein 5ng
Lane 3: Recombinant Mouse MMP-7 Protein 2.5ng
Anti-MMP-7 Antibody
Anti-MMP7 antibody, PA1471, IHC(P)
IHC(P): Rat Liver Tissue
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Yang B, Gao J, Rao Z, Zhang B, Ouyang W, Yang C. Ultrastruct Pathol. 2011 Dec;35(6):256-9. Doi: 10.3109/01913123.2011.610564. Epub 2011 Oct 7. Antisense Oligonucleotide Targeting Matrix Metalloproteinase-7 (Mmp-7) Changes The Ultrastructure Of Hum...
Pan Fy, Zhang Sz, Xu N, Meng Fl, Zhang Hx, Xue B, Han X, Li Cj. Histochem Cell Biol. 2010 Sep;134(3):285-95. Doi: 10.1007/S00418-010-0729-3. Epub 2010 Jul 31. Beta-Catenin Signaling Involves Hgf-Enhanced Hepg2 Scattering Through Activating Mmp-7 T...
Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mmp 7 antibody, mmp7 antibody, mmp-7 antibody