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SKU:PB9037
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:ELISA, IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-MMP-7 Antibody
SKU/Catalog Number PB9037
Description Rabbit IgG polyclonal antibody for Matrilysin(MMP7) detection. Tested with WB, IHC-P, ELISA in Human.
Cite This Product Anti-MMP-7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9037)
Replacement Item This antibody may replace the following items: sc-101566|sc-26674|sc-30071|sc-58388|sc-80205|sc-8832 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application ELISA, IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human MMP7 recombinant protein (Position: M1-K267). Human MMP7 shares 71% amino acid (aa) sequence identity with both mouse and rat MMP7.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MMP7
Protein Name Matrilysin
Molecular Weight 29677 MW
Protein Function Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. .
Sequence Similarities Belongs to the peptidase M10A family.
Subcellular Localization Secreted, extracellular space, extracellular matrix .
Uniprot ID P09237
Alternative Names Matrilysin;3.4.24.23;Matrin;Matrix metalloproteinase-7;MMP-7;Pump-1 protease;Uterine metalloproteinase;MMP7;MPSL1, PUMP1;
Research Areas |cardiovascular|angiogenesis|adhesion / ecm|matrix metalloproteinases|mmp| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes| cancer|invasion/microenvironment|mmps| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Matrilysin

Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The MMP-7 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The MMP-7 protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. MMP-7, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, MMP-7 mRNA is regulated in a specific and distinct manner in normal and malignant tissues. MMP-7 has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined. MMP-7 is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.

Anti-MMP-7 Antibody Images

Click the images to enlarge.

Anti-MMP-7 Antibody
Anti-MMP7 Picoband antibody, PB9037-1.JPG
IHC(P): Human Intestinal Cancer Tissue
Anti-MMP-7 Antibody
Anti-MMP7 Picoband antibody, PB9037-2.JPG
IHC(P): Human Placenta Tissue
Anti-MMP-7 Antibody
Anti-MMP7 Picoband antibody, PB9037-3.jpg
All lanes: Anti-MMP7(PB9037) at 0.5ug/ml
WB: A549 Whole Cell Lysate at 40ug
Predicted bind size: 30KD
Observed bind size: 30KD
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Publications

Yang B, Gao J, Rao Z, Zhang B, Ouyang W, Yang C. Ultrastruct Pathol. 2011 Dec;35(6):256-9. Doi: 10.3109/01913123.2011.610564. Epub 2011 Oct 7. Antisense Oligonucleotide Targeting Matrix Metalloproteinase-7 (Mmp-7) Changes The Ultrastructure Of Hum...
Pan Fy, Zhang Sz, Xu N, Meng Fl, Zhang Hx, Xue B, Han X, Li Cj. Histochem Cell Biol. 2010 Sep;134(3):285-95. Doi: 10.1007/S00418-010-0729-3. Epub 2010 Jul 31. Beta-Catenin Signaling Involves Hgf-Enhanced Hepg2 Scattering Through Activating Mmp-7 T...
Yang Y, Shi H, Li X, Yi Y. Oncol Rep. 2011 Jan;25(1):259-65. Effects Of Shrna Targeting Maspin On Invasion Of Gastric Carcinoma Sgc7901 Cell Line.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mmp 7 antibody, mmp7 antibody, mmp-7 antibody