Data & Images
|Product Name||Anti-Monocarboxylic Acid Transporter 1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Monocarboxylate transporter 1(SLC16A1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-Monocarboxylic Acid Transporter 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2188)|
|Replacement Item||This antibody may replace the following items: sc-100477|sc-14916|sc-14917|sc-365501|sc-366137|sc-390858|sc-50324|sc-86725|sc-86727 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Monocarboxylic acid transporter 1(236-254aa RHPKQEKRSVFQTINQFLD).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Monocarboxylate transporter 1|
|Molecular Weight||53944 MW|
|Protein Function||Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis. .|
|Tissue Specificity||Detected in heart and in blood lymphocytes and monocytes (at protein level). Widely expressed. .|
|Sequence Similarities||Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.|
|Subcellular Localization||Cell membrane ; Multi- pass membrane protein .|
|Alternative Names||Monocarboxylate transporter 1;MCT 1;Solute carrier family 16 member 1;SLC16A1;MCT1;|
Background for Monocarboxylate transporter 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Monocarboxylic Acid Transporter 1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Monocarboxylic Acid Transporter 1 Antibody Images
Click the images to enlarge.
Lane 1: COLO320 Cell Lysate
Lane 2: SKOV Cell Lysate
Lane 3: A549 Cell Lysate
IHC(P): Human Rectal Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,