Anti-Mouse CD3E DyLight® 488 conjugated Antibody

Cd3e antibody

Boster Bio Anti-Mouse CD3E DyLight® 488 conjugated Antibody catalog # A02675-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Mouse.

Product Info Summary

SKU: A02675-Dyl488
Size: 50ug/vial
Reactive Species: Mouse
Host: Rabbit
Application: Flow Cytometry

Product Name

Anti-Mouse CD3E DyLight® 488 conjugated Antibody

View all Cd3e Antibodies

SKU/Catalog Number

A02675-Dyl488

Size

50ug/vial

Form

Liquid

Description

Boster Bio Anti-Mouse CD3E DyLight® 488 conjugated Antibody catalog # A02675-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Mouse CD3E DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02675-Dyl488)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN4.

Clonality

Polyclonal

Immunogen

E.coli-derived mouse CD3 epsilon/Cd3e recombinant protein (Position: D22-A113).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A02675-Dyl488 is reactive to Cd3e in Mouse

Applications

A02675-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Background of Cd3e

CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells, mouse

Validation Images & Assay Conditions

Gene/Protein Information For Cd3e (Source: Uniprot.Org, NCBI)

Uniprot ID

P22646

Gene ID

12501

Gene Name

Cd3e

Full Name

T-cell surface glycoprotein CD3 epsilon chain

Weight

21.393kDa

Alternative Names

CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain Cd3e|AI504783, CD3, CD3epsilon, T3e|CD3 antigen, epsilon polypeptide|T-cell surface glycoprotein CD3 epsilon chain|T-cell surface antigen T3/Leu-4 epsilon chain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd3e, check out the Cd3e Infographic

Cd3e infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cd3e: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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3 Customer Q&As for Anti-Mouse CD3E DyLight® 488 conjugated Antibody

Question

Our lab were satisfied with the WB result of your anti-Mouse CD3E DyLight® 488 conjugated antibody . However we have observed positive staining in thymus cell membrane using this antibody. Is that expected? Could you tell me where is CD3E supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

Based on literature, thymus does express CD3E. Generally CD3E expresses in cell membrane. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-04-16

Question

We are currently using anti-Mouse CD3E DyLight® 488 conjugated antibody A02675-Dyl488 for mouse tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on goat tissues as well?

A. Collins

Verified customer

Asked: 2020-03-12

Answer

The anti-Mouse CD3E DyLight® 488 conjugated antibody (A02675-Dyl488) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-12

Question

We have seen staining in mouse blood. Do you have any suggestions? Is anti-Mouse CD3E DyLight® 488 conjugated antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

From literature blood does express CD3E. From Uniprot.org, CD3E is expressed in leukocyte, blood, thymus, leukemic t-cell, among other tissues. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-06-07

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A02675-Dyl488
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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