Product Info Summary
SKU: | A08340 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-MPP1/KIF20B Antibody Picoband™
SKU/Catalog Number
A08340
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MPP1/KIF20B Antibody Picoband™ catalog # A08340. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MPP1/KIF20B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08340)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MPP1/KIF20B recombinant protein (Position: A19-D231).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08340 is reactive to KIF20B in Human, Mouse, Rat
Applications
A08340 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
211 kDa
Calculated molecular weight
210.629kDa
Background of MPHOSPH1
Kinesin-like protein KIF20B is a protein that in humans is encoded by the KIF20B gene. The MPP1 gene is previously labeled as KIF20B. Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Boster Kit Box
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Figure 1. Western blot analysis of KIF20B using anti-KIF20B antibody (A08340).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF20B antigen affinity purified polyclonal antibody (Catalog # A08340) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIF20B at approximately 211KD. The expected band size for KIF20B is at 211KD.
Protein Target Info & Infographic
Gene/Protein Information For KIF20B (Source: Uniprot.org, NCBI)
Gene Name
KIF20B
Full Name
Kinesin-like protein KIF20B
Weight
210.629kDa
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
Cancer/testis antigen 90; CT90MPP-1; DKFZp434B0435; EC 6.3.5.5; kinesin family member 20B; Kinesin-related motor interacting with PIN1; KRMP1kinesin-like protein KIF20B; M-phase phosphoprotein 1mitotic kinesin-like protein; MPHOSPH1EC 2.1.1; MPP1DKFZp434P0810 KIF20B CT90, KRMP1, MPHOSPH1, MPP-1, MPP1 kinesin family member 20B kinesin-like protein KIF20B|M-phase phosphoprotein 1|cancer/testis antigen 90|kinesin-related motor interacting with PIN1|mitotic kinesin-like protein|mitotic kinesin-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KIF20B, check out the KIF20B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KIF20B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MPP1/KIF20B Antibody Picoband™ (A08340)
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No publications found for A08340
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Customer Q&As
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1 Customer Q&As for Anti-MPP1/KIF20B Antibody Picoband™
Question
We are currently using anti-MPP1/KIF20B antibody A08340 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-29
Answer
The anti-MPP1/KIF20B antibody (A08340) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-29