|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-MRGX1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Mas-related G-protein coupled receptor member X1(MRGPRX1) detection. Tested with WB, IHC-P in Rat.|
|Cite This Product||Anti-MRGX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1208)|
|Replacement Item||This antibody may replace the following items: sc-7910|sc-1884|sc-4572|sc-13123|sc-1359|sc-55520|sc-13123|sc-1359|sc-1884|sc-4572|sc-55520|sc-7910 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of rat MRGX1(14-28aa TLNKTGHPSCRPILT).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mas-related G-protein coupled receptor member X1|
|Molecular Weight||37074 MW|
|Protein Function||Orphan receptor activated by neuropeptides terminating in Arg-Phe or Arg-Phe-amide. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. May regulate the function of nociceptive neurons by modulation of pain perception (By similarity). .|
|Tissue Specificity||Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons. Associated preferentially with IB4 class of small-diameter somatosensory afferents (also known as nociceptors). .|
|Sequence Similarities||Belongs to the G-protein coupled receptor 1 family. Mas subfamily.|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Alternative Names||Mas-related G-protein coupled receptor member X1;Sensory neuron-specific G-protein coupled receptor 1;Mrgprx1;Mrgc, Mrgprc, Mrgprc11, Snsr, Snsr1;|
|Research Areas|||signal transduction|signaling pathway|g protein signaling|gpcr||
Background for Mas-related G-protein coupled receptor member X1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MRGX1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MRGX1 Antibody Images
Click the images to enlarge.
Lane 1: Marker
Lane 2: Rat Heart Tissue Lysate
Lane 3: Rat Skeletal Muscle Tissue Lysate
Lane 4: MCF-7 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,