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SKU:PA1208
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-MRGX1 Antibody
SKU/Catalog Number PA1208
Description Rabbit IgG polyclonal antibody for Mas-related G-protein coupled receptor member X1(MRGPRX1) detection. Tested with WB, IHC-P in Rat.
Cite This Product Anti-MRGX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1208)
Replacement Item This antibody may replace the following items: sc-7910|sc-1884|sc-4572|sc-13123|sc-1359|sc-55520|sc-13123|sc-1359|sc-1884|sc-4572|sc-55520|sc-7910 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of rat MRGX1(14-28aa TLNKTGHPSCRPILT).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Mrgprx1
Protein Name Mas-related G-protein coupled receptor member X1
Molecular Weight 37074 MW
Protein Function Orphan receptor activated by neuropeptides terminating in Arg-Phe or Arg-Phe-amide. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. May regulate the function of nociceptive neurons by modulation of pain perception (By similarity). .
Tissue Specificity Uniquely localized in a subset of small dorsal root and trigeminal sensory neurons. Associated preferentially with IB4 class of small-diameter somatosensory afferents (also known as nociceptors). .
Sequence Similarities Belongs to the G-protein coupled receptor 1 family. Mas subfamily.
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Uniprot ID Q8R4G1
Alternative Names Mas-related G-protein coupled receptor member X1;Sensory neuron-specific G-protein coupled receptor 1;Mrgprx1;Mrgc, Mrgprc, Mrgprc11, Snsr, Snsr1;
Research Areas |signal transduction|signaling pathway|g protein signaling|gpcr|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Mas-related G-protein coupled receptor member X1

MAS-related G-protein coupled receptor, member C, also known as MRGPRC or MRGPRX1, is a human gene. The Mas-related genes(Mrgs) comprise a family of >50 G protein-coupled receptors(GPCRs), many of which are expressed in specific subsets of nociceptive sensory neurons in mice. In contrast, humans contain a related but nonorthologous family of genes, called MrgXs or sensory neuron-specific receptors, of which many fewer appear to be expressed in sensory neurons.

Anti-MRGX1 Antibody Images

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Anti-MRGX1 Antibody
Anti-MRGX1 antibody, PA1208, Western blotting
Lane 1: Marker
Lane 2: Rat Heart Tissue Lysate
Lane 3: Rat Skeletal Muscle Tissue Lysate
Lane 4: MCF-7 Cell Lysate
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.