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SKU:PB9144
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-MRP4 Picoband™ Antibody
SKU/Catalog Number PB9144
Description Rabbit IgG polyclonal antibody for Multidrug resistance-associated protein 4(ABCC4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-MRP4 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9144)
Replacement Item This antibody may replace the following items: sc-20768|sc-376262|sc-5765|sc-5767|sc-59614 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human MRP4 recombinant protein (Position: M1-P370).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ABCC4
Protein Name Multidrug resistance-associated protein 4
Molecular Weight 149527 MW
Protein Function May be an organic anion pump relevant to cellular detoxification.
Tissue Specificity Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
Sequence Similarities Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Subcellular Localization Membrane; Multi-pass membrane protein.
Uniprot ID O15439
Alternative Names Multidrug resistance-associated protein 4;ATP-binding cassette sub-family C member 4;MRP/cMOAT-related ABC transporter;Multi-specific organic anion transporter B;MOAT-B;ABCC4;MRP4;
Research Areas |signal transduction|metabolism|plasma membrane|atpases| signal transduction|channels|drug metabolism| cancer|drug resistance|mrp-related proteins| metabolism|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Multidrug resistance-associated protein 4

ABCC4(Atp-binding cassette, subfamily c, member 4) also known as MRP4 or MOATB, is a protein that in humans is encoded by the ABCC4 gene. It belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. This gene is mapped to chromosome 13q32. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus. The antiproliferative effect of ABCC4 inhibition was related to cAMP-dependent PKA activation and CREB phosphorylation. Pharmacologic inhibition of ABCC4 activity or knockdown of ABCC4 via RNA interference resulted in reduced migration of DCs from human skin explants and of in vitro-generated Langerhans cells. It has been found that ABCC4 contributes to migration of DCs toward draining lymph nodes and therefore has a role in the initiation of an immune response.

Anti-MRP4 Picoband™ Antibody Images

Click the images to enlarge.

Anti-MRP4 Picoband™ Antibody
Anti-MRP4 Picoband antibody, PB9144-1.jpg
All lanes: Anti MRP4 (PB9144) at 0.5ug/ml
WB: Recombinant Human MRP4 Protein 0.5ng
Predicted bind size: 47KD
Observed bind size: 47KD
Anti-MRP4 Picoband™ Antibody
Anti-MRP4 Picoband antibody, PB9144-2.JPG
IHC(P): Rat Intestine Tissue
Anti-MRP4 Picoband™ Antibody
Anti-MRP4 Picoband antibody, PB9144-3.JPG
IHC(P): Human Intestinal Cancer Tissue
Anti-MRP4 Picoband™ Antibody
Anti-MRP4 Picoband antibody, PB9144-4.JPG
IHC(P): Mouse Intestine Tissue
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is abcc4 antibody