Anti-mTOR/Tor Antibody

TOR/mTOR antibody

Boster Bio Anti-mTOR/Tor Antibody catalog # A00003-1. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00003-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-mTOR/Tor Antibody

View all TOR/mTOR Antibodies

SKU/Catalog Number

A00003-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-mTOR/Tor Antibody catalog # A00003-1. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-mTOR/Tor Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00003-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

6G1

Isotype

IgG

Immunogen

Synthesized peptide derived from human mTOR around the non-phosphorylation site of S2448.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00003-1 is reactive to MTOR in Human, Mouse, Rat

Applications

A00003-1 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

288.892kDa

Background of TOR/mTOR

CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MTOR (Source: Uniprot.org, NCBI)

Gene Name

MTOR

Full Name

Serine/threonine-protein kinase mTOR

Weight

288.892kDa

Superfamily

PI3/PI4-kinase family

Alternative Names

EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/thr MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS mechanistic target of rapamycin kinase serine/threonine-protein kinase mTOR|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1|mammalian target of rapamycin|mechanistic target of rapamycin (serine/threonine kinase)|rapamycin and FKBP12 target 1|rapamycin associated protein FRAP2|rapamycin target protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MTOR, check out the MTOR Infographic

MTOR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MTOR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00003-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lu Kong,Yongya Wu,Wangcheng Hu,Lin Liu,Yuying Xue,Geyu Liang,Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells,Environmental Pollution,2021,116556,ISSN 0269-7
Species: Mouse
A00003-1 usage in article: APP:WB, SAMPLE:GC-1 CELL, DILUTION:1:1000

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16 Customer Q&As for Anti-mTOR/Tor Antibody

Question

My question regards using your anti-mTOR/Tor antibody for smith-kingsmore syndrome (sks) studies. Has this antibody been tested with western blotting on huvec cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-14

Answer

We appreciate your inquiry. This A00003-1 anti-mTOR/Tor antibody is tested on huvec cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-14

Question

Is this A00003-1 anti-mTOR/Tor antibody reactive to the isotypes of MTOR?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

The immunogen of A00003-1 anti-mTOR/Tor antibody is Synthesized peptide derived from human mTOR around the non-phosphorylation site of S2448. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-25

Question

We are currently using anti-mTOR/Tor antibody A00003-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

The anti-mTOR/Tor antibody (A00003-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-16

Question

We ordered your anti-mTOR/Tor antibody for WB on cervix carcinoma in the past. I am using human, and I plan to use the antibody for IF next. My question regards examining cervix carcinoma as well as cerebellum in our next experiment. Could give a recommendation on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

I have checked the website and datasheets of our anti-mTOR/Tor antibody and it seems that A00003-1 has been tested on human in both WB and IF. Thus A00003-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-28

Question

Will A00003-1 anti-mTOR/Tor antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

It shows on the product datasheet, A00003-1 anti-mTOR/Tor antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-15

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-mTOR/Tor antibody A00003-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-15

Question

We have been able to see staining in mouse cervix carcinoma erythroleukemia. What should we do? Is anti-mTOR/Tor antibody supposed to stain cervix carcinoma erythroleukemia positively?

Verified Customer

Verified customer

Asked: 2018-01-15

Answer

Based on literature cervix carcinoma erythroleukemia does express MTOR. Based on Uniprot.org, MTOR is expressed in testis, brain, cerebellum, b-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2018-01-15

Question

I was wanting to use to test anti-mTOR/Tor antibody A00003-1 on mouse testis for research purposes, then I may be interested in using anti-mTOR/Tor antibody A00003-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-11-28

Answer

The products we sell, including anti-mTOR/Tor antibody A00003-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-28

Question

I have a question about product A00003-1, anti-mTOR/Tor antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-11-03

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00003-1 anti-mTOR/Tor antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-11-03

Question

We were happy with the WB result of your anti-mTOR/Tor antibody. However we have seen positive staining in b-cell endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is MTOR supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-09-12

Answer

According to literature, b-cell does express MTOR. Generally MTOR expresses in endoplasmic reticulum membrane. Regarding which tissues have MTOR expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 7809080
Brain, Pubmed ID: 8008069
Cerebellum, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2017-09-12

Question

Would anti-mTOR/Tor antibody A00003-1 work for IHC with testis?

Verified Customer

Verified customer

Asked: 2017-08-03

Answer

According to the expression profile of testis, MTOR is highly expressed in testis. So, it is likely that anti-mTOR/Tor antibody A00003-1 will work for IHC with testis.

Boster Scientific Support

Answered: 2017-08-03

Question

I was wanting to use your anti-mTOR/Tor antibody for IHC for mouse testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse testis identification?

Verified Customer

Verified customer

Asked: 2017-06-16

Answer

It shows on the product datasheet, A00003-1 anti-mTOR/Tor antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-06-16

Question

Is a blocking peptide available for product anti-mTOR/Tor antibody (A00003-1)?

T. Anderson

Verified customer

Asked: 2014-02-13

Answer

We do provide the blocking peptide for product anti-mTOR/Tor antibody (A00003-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-02-13

Question

Here is the WB image, lot number and protocol we used for testis using anti-mTOR/Tor antibody A00003-1. Please let me know if you require anything else.

R. Moore

Verified customer

Asked: 2014-01-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-01-06

Question

I see that the anti-mTOR/Tor antibody A00003-1 works with IHC, what is the protocol used to produce the result images on the product page?

D. Johnson

Verified customer

Asked: 2013-08-08

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-08-08

Question

Is there a BSA free version of anti-mTOR/Tor antibody A00003-1 available?

A. Krishna

Verified customer

Asked: 2013-04-15

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-mTOR/Tor antibody A00003-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-04-15

Order DetailsPrice
A00003-1

100uL

$399

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