|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-mtTFA Antibody|
|Description||Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.|
|Cite This Product||Anti-mtTFA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1936)|
|Replacement Item||This antibody may replace the following items: sc-19050|sc-19050-X|sc-28200|sc-28200-X|sc-30962|sc-30963|sc-376672 from Santa Cruz Biotechnology.|
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human mtTFA(227-246aa RKDLLRRTIKKQRKYGAEEC).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Transcription factor A, mitochondrial|
|Molecular Weight||29097 MW|
|Protein Function||Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. .|
|Subcellular Localization||Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.|
|Alternative Names||Transcription factor A, mitochondrial;mtTFA;Mitochondrial transcription factor 1;MtTF1;Transcription factor 6;TCF-6;Transcription factor 6-like 2;TFAM;TCF6, TCF6L2;|
|Research Areas|||tags & cell markers|subcellular markers|organelles|mitochondria| epigenetics and nuclear signaling|transcription|transcription factors|chromatin binding proteins|dna / rna binding| metabolism|pathways and processes|metabolic signaling pathways|nucleotide metabolism|molecular processes|mitochondrial transcription|mitochondrial metabolism|mitochondrial markers||
Background for Transcription factor A, mitochondrial
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-mtTFA Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-mtTFA Antibody Images
Click the images to enlarge.
Lane 1: HELA Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: 293T Cell Lysate
Lane 4: Rabbit igG(55kD)
Lane 5: A431 Cell Lysate
Lane 6: RAJI Cell Lysate
Lane 7: CEM Cell Lysate
Lane 8: HL-60 Cell Lysate
Lane 9: HUT Cell Lysate
IHC(P): Human Intestinal Cancer Tissue
ICC: HELA Cell
IHC(F): Human Placenta Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,