|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-MUC1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Mucin-1(MUC1) detection. Tested with WB, IHC-P, IHC-F in Mouse;Rat.|
|Cite This Product||Anti-MUC1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9112)|
|Replacement Item||This antibody may replace the following items: sc-99596|sc-99595|sc-100652 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived mouse MUC1 recombinant protein (Position: S474-L630). Mouse MUC1 shares 87% and amino acid (aa) sequence identity with human MUC1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||64539 MW|
|Protein Function||The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.|
|Tissue Specificity||Expressed in a variety of epithelial tissues.|
|Sequence Similarities||Contains 1 SEA domain.|
|Subcellular Localization||Apical cell membrane; Single-pass type I membrane protein. Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions (By similarity). .|
|Alternative Names||Mucin-1;MUC-1;Episialin;CD227;Mucin-1 subunit alpha;MUC1-NT;MUC1-alpha;Mucin-1 subunit beta;MUC1-beta;MUC1-CT;Muc1;Muc-1;|
|Research Areas||tags & cell markers|cell type markers|tumor associated|signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|muc|cancer|invasion/microenvironment|tumor immunology|tumor-associated antigens||
Background for Mucin-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MUC1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Mouse, Rat, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MUC1 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti MUC1 (PB9112) at 0.5ug/ml
WB: RH35 Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 122KD
IHC(P): Rat Kidney Tissue
IHC(P): Mouse Lung Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,