Data & Images
|Product Name||Anti-MUC1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Mucin-1(MUC1) detection. Tested with IHC-P in Human.|
|Cite This Product||Anti-MUC1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9145)|
|Replacement Item||This antibody may replace the following items: sc-130842|sc-15333|sc-25274|sc-27260|sc-367829|sc-52089|sc-52092|sc-52093|sc-52094|sc-52347|sc-53376|sc-53377|sc-53381|sc-59798|sc-6825|sc-6827|sc-69644|sc-71613|sc-71615|sc-7313 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human MUC1 recombinant protein (Position: R945-G1097). Human MUC1 shares 56% amino acid (aa) sequence identity with mouse MUC1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||68996 MW|
|Protein Function||Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. .|
|Tissue Specificity||Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only.|
|Sequence Similarities||Contains 1 SEA domain.|
|Subcellular Localization||Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.|
|Alternative Names||Prostaglandin G/H synthase 2;18.104.22.168;Cyclooxygenase-2;COX-2;PHS II;Prostaglandin H2 synthase 2;PGH synthase 2;PGHS-2;Prostaglandin-endoperoxide synthase 2;PTGS2;COX2;|
|Research Areas|||tags & cell markers|cell type markers|tumor associated| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|muc| cancer|invasion/microenvironment|tumor immunology|tumor-associated antigens||
Background for Mucin-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MUC1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MUC1 Picoband™ Antibody Images
Click the images to enlarge.
IHC(P): Human Ovary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,