Data & Images
|Product Name||Anti-MUC2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Mucin-2(MUC2) detection. Tested with IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-MUC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1038)|
|Replacement Item||This antibody may replace the following items: sc-13312|sc-15334|sc-23170|sc-23171|sc-515032|sc-515106|sc-59859|sc-59956|sc-7314 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human MUC2 recombinant protein (Position: S21-W380). Human MUC2 shares 78% and 79% amino acid (aa) sequences identity with mouse and rat MUC2, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||540300 MW|
|Protein Function||Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. .|
|Tissue Specificity||Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.|
|Subcellular Localization||Secreted. In the intestine, secreted into the inner and outer mucus layers. .|
|Alternative Names||Mucin-2;MUC-2;Intestinal mucin-2;MUC2;SMUC;|
|Research Areas|||tags & cell markers|cell type markers|tumor associated| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|muc| cancer|invasion/microenvironment|tumor immunology|tumor-associated antigens||
Background for Mucin-2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MUC2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MUC2 Antibody Images
Click the images to enlarge.
IHC(P): Human Gastric Cancer Tissue
IHC(P): Mouse Intestine Tissue
IHC(P): Rat Intestine Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,