|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-Mucin Gastric Antibody (Monoclonal, 45M1)|
|Description||Mouse IgG monoclonal antibody for Mucin Gastric, mucin 5AC, oligomeric mucus/gel-forming (MUC5AC) detection. Tested with WB, IHC-P, ICC in Human;mouse;rabbit;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-Mucin Gastric Antibody (Monoclonal, 45M1) 45M1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1061)|
|Replacement Item||This antibody may replace the following items: sc-16910|sc-20118|sc-21701|sc-33667|sc-398985|sc-59950|sc-59951|sc-71620|sc-71621 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit, Rat|
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Mucin from human ovarian cyst fluid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||585570 MW|
|Protein Function||Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.|
|Tissue Specificity||Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .|
|Sequence Similarities||Contains 1 CTCK (C-terminal cystine knot-like) domain.|
|Subcellular Localization||Secreted .|
|Alternative Names||Mucin-5AC ;MUC-5AC ;Gastric mucin ;Lewis B blood group antigen ;LeB ;Major airway glycoprotein ;Mucin-5 subtype AC, tracheobronchial;Tracheobronchial mucin ;TBM ;MUC5AC ;MUC5 ;|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|muc| cell biology|other antibodies||
Background for Mucin-5AC
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Mucin Gastric Antibody (Monoclonal, 45M1) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 2-4μg/ml, Human, mouse, rabbit, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rabbit, rat, -
Western blot, 1-2μg/ml, Human, mouse, rabbit, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Mucin Gastric Antibody (Monoclonal, 45M1) Images
Click the images to enlarge.
IHC(P): Human Gastric Cancer Tissue
IHC(P): Rat Gastric Cancer Tissue