Show Order Info
SKU:MA1061
Clonality:Monoclonal
Clone Number:45M1
Application:IHC-P, ICC, WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-Mucin Gastric Antibody (Monoclonal, 45M1)
Description Mouse IgG monoclonal antibody for Mucin Gastric, mucin 5AC, oligomeric mucus/gel-forming (MUC5AC) detection. Tested with WB, IHC-P, ICC in Human;mouse;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-Mucin Gastric Antibody (Monoclonal, 45M1) 45M1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1061)
Replacement Item This antibody may replace the following items: sc-16910|sc-20118|sc-21701|sc-33667|sc-398985|sc-59950|sc-59951|sc-71620|sc-71621 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Mouse, Rabbit, Rat
Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Mucin from human ovarian cyst fluid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number 45M1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MUC5AC
Protein Name Mucin-5AC
Molecular Weight 585570 MW
Protein Function Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
Tissue Specificity Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .
Sequence Similarities Contains 1 CTCK (C-terminal cystine knot-like) domain.
Subcellular Localization Secreted .
Uniprot ID P98088
Alternative Names Mucin-5AC ;MUC-5AC ;Gastric mucin ;Lewis B blood group antigen ;LeB ;Major airway glycoprotein ;Mucin-5 subtype AC, tracheobronchial;Tracheobronchial mucin ;TBM ;MUC5AC ;MUC5 ;
Research Areas |signal transduction|cytoskeleton / ecm|extracellular matrix|ecm proteins|muc| cell biology|other antibodies|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Mucin-5AC

Mucin 5AC (Muc5AC) is a protein that in humans is encoded by the MUC5AC gene. Using real-time RT-PCR, Moehle et al. (2006) found that MUC5AC was highly expressed in adult trachea and stomach. Expression was lower in adult lung and weak in fetal lung, and no expression was detected in other tissues. This gene has been linked to mucus hypersecretion in the pulmonary tracts and associated to chronic obstructive pulmonary disease. Moreover, Muc5ac is identified as a direct and critical mediator of resistance during intestinal nematode infection.

Anti-Mucin Gastric Antibody (Monoclonal, 45M1) Images

Click the images to enlarge.

Anti-Mucin Gastric Antibody (Monoclonal, 45M1)
Anti- Mucin Gastric antibody, MA1061, IHC(P)
IHC(P): Human Gastric Cancer Tissue
Anti-Mucin Gastric Antibody (Monoclonal, 45M1)
Anti- Mucin Gastric antibody, MA1061, IHC(P)
IHC(P): Rat Gastric Cancer Tissue
Write a review for MA1061

Publications

Zhou J, Perelman Jm, Kolosov Vp, Zhou X. Mol Cell Biochem. 2013 May;377(1-2):75-85. Doi: 10.1007/S11010-013-1572-3. Epub 2013 Feb 8. Neutrophil Elastase Induces Muc5Ac Secretion Via Protease-Activated Receptor 2.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is muc6 antibody