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SKU:PA1050
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Myelin Basic Protein Antibody
SKU/Catalog Number PA1050
Description Rabbit IgG polyclonal antibody for Myelin basic protein(MBP) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Myelin Basic Protein Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1050)
Replacement Item This antibody may replace the following items: sc-31527|sc-31528|sc-31526|sc-25665|sc-271524|sc-13912|sc-13914|sc-376995|sc-58542|sc-66064|sc-71546|sc-398212|sc-13914-R from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein(182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MBP
Protein Name Myelin basic protein(MBP)
Molecular Weight 33117 MW
Protein Function The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. .
Tissue Specificity MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. .
Sequence Similarities Belongs to the myelin basic protein family.
Subcellular Localization Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic side of myelin.
Uniprot ID P02686
Alternative Names Myelin basic protein;MBP;Myelin A1 protein;Myelin membrane encephalitogenic protein;MBP;
Research Areas |tags & cell markers|fusion / marker proteins|mbp|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Myelin basic protein(MBP)

Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Anti-Myelin Basic Protein Antibody Images

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Anti-Myelin Basic Protein Antibody
Anti-Myelin Basic Protein antibody, PA1050, Western blotting
WB: Mouse Brain Tissue Lysate
Anti-Myelin Basic Protein Antibody
Anti-Myelin Basic Protein antibody, PA1050, IHC(P)
IHC(P): Rat Brain Tissue
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Publications

Gao S, Zheng Y, Cai Q, Yao W, Wang J, Zhang P, Wang X. J Mater Sci Mater Med. 2014 May;25(5):1283-91. Doi: 10.1007/S10856-014-5150-3. Epub 2014 Jan 23. Comparison Of Morphology And Biocompatibility Of Acellular Nerve Scaffolds Processed By Differe...
Wang B, Xiao Z, Chen B, Han J, Gao Y, Zhang J, Zhao W, Wang X, Dai J. Plos One. 2008 Mar 26;3(3):E1856. Doi: 10.1371/Journal.Pone.0001856. Nogo-66 Promotes The Differentiation Of Neural Progenitors Into Astroglial Lineage Cells Through Mtor-Stat3 ...
Jin Sj, Liu Y, Deng Sh, Liao Lh, Lin Tl, Ning Q, Luo Xp. Int J Mol Med. 2015 May;35(5):1199-212. Doi: 10.3892/Ijmm.2015.2136. Epub 2015 Mar 13. Neuroprotective Effects Of Activated Protein C On Intrauterine Inflammation-Induced Neonatal White Matt...
Wei J, Zheng M, Liang P, Wei Y, Yin X, Tang Y, Xue Y. Neurobiol Dis. 2013 Aug;56:59-65. Doi: 10.1016/J.Nbd.2013.04.009. Epub 2013 Apr 22. Apolipoprotein E And Its Mimetic Peptide Suppress Th1 And Th17 Responses In Experimental Autoimmune Encephalo...
Chen X,, Chen X, Huang X, Qin C, Fang Y, Liu Y, Zhang G, Pan D, Wang W, Xie M. Mol Neurobiol. 2015 Feb 10. [Epub Ahead Of Print] Soluble Epoxide Hydrolase Inhibition Provides Multi-Target Therapeutic Effects In Rats After Spinal Cord Injury.
Han Sm, Wan H, Kudo G, Foltz Wd, Vines Dc, Green De, Zoerle T, Tariq A, Brathwaite S, D'Abbondanza J, Ai J, Macdonald Rl. J Cereb Blood Flow Metab. 2014 Jan;34(1):108-17. Doi: 10.1038/Jcbfm.2013.170. Epub 2013 Sep 25. Molecular Alterations In The ...
Wang X, Tu Q, Zhao B, An Y, Wang Jc, Liu W, Yuan Ms, Ahmed Sm, Xu J, Liu R, Zhang Y, Wang J. Biomaterials. 2013 Jan;34(4):1155-69. Doi: 10.1016/J.Biomaterials.2012.10.063. Epub 2012 Nov 17. Effects Of Poly(L-Lysine)-Modified Fe3O4 Nanoparticles On...
Ping Yf, Yao Xh, Jiang Jy, Zhao Lt, Yu Sc, Jiang T, Lin Mc, Chen Jh, Wang B, Zhang R, Cui Yh, Qian C, Wang Jm, Bian Xw. J Pathol. 2011 Jul;224(3):344-54. Doi: 10.1002/Path.2908. Epub 2011 May 27. The Chemokine Cxcl12 And Its Receptor Cxcr4 Promote...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to mbp antibody, myelin basic protein antibody, sp antibody