SKU A00372
Size 2mL
Reactivity Human
Clonality Polyclonal
Host Rabbit
Applications IP, WB

Overview

Product Name Anti-Myeloperoxidase Antibody
SKU/Catalog Number A00372
Storage & Handling [storage]
Size 2mL
Description Rabbit Polyclonal antibody for MPO detection. Tested positive for ELISA,Immunoprecipitation,Western Blot, in human
Cite This Product Anti-Myeloperoxidase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00372)
Host Rabbit
Contents/Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form Lyophilized
Immunogen Myeloperoxidase [Human Leukocytes]
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Western Blot: 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Images And Assay Conditions

/A/0/A00372 MPO WB test 1.jpg

Figure 1. Western blot analysis of MPO using anti-MPO antibody (A00372).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # A00372) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MPO.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P05164
Gene Name MPO

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Cancer, Cancer Metabolism, Cardiovascular, Immunology, Innate Immunity, Macrophage / Inflamm., Metabolism, Pathways And Processes, Redox Metabolism, Vasculature

*You can search these to find other products in these research areas.

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Order Product (A00372)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
2mL $1019
2mL+Free HRP Secondary BA1054 $1019
2mL+Free Biotin Secondary BA1003 $1019

USD $1,019

Ships in 7-10 business days.

Troubleshooting

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Publications

The Gastric Mucosa from Patients Infected with CagA or VacA Helicobacter pylori Has a Lower Level of Dual Oxidase-2 Expression than Uninfected or %u2026