SKU PB9057
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-Myeloperoxidase/MPO Picoband™ Antibody
SKU/Catalog Number PB9057
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Myeloperoxidase(MPO) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Myeloperoxidase/MPO Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9057)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen E.coli-derived human Myeloperoxidase recombinant protein (Position:S406-S745). Human Myeloperoxidase shares 90% amino acid (aa) sequence identity with mouse Myeloperoxidase.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

/antibody/pb9057 1 IHC anti mpo myeloperoxidase antibody.jpg

Anti-Myeloperoxidase Picoband antibody, PB9057-1.JPG
IHC(P): Human Appendicitis Tissue

/antibody/pb9057 2 IHC anti mpo myeloperoxidase antibody.jpg

Anti-Myeloperoxidase Picoband antibody, PB9057-2.JPG
IHC(P): Mouse Spleen Tissue

/antibody/pb9057 3 IHC anti mpo myeloperoxidase antibody.jpg

Anti-Myeloperoxidase Picoband antibody, PB9057-3.JPG
IHC(P): Rat Spleen Tissue

/antibody/pb9057 4 WB anti mpo myeloperoxidase antibody.jpg

Anti-Myeloperoxidase Picoband antibody, PB9057-4.jpg
All lanes: Anti- Myeloperoxidase(PB9057) at 0.5ug/ml
WB: Recombinant Human Myeloperoxidase Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P05164
Gene Name MPO
Protein Name Myeloperoxidase
Alternative Names Myeloperoxidase;MPO;1.11.2.2;Myeloperoxidase;89 kDa myeloperoxidase;84 kDa myeloperoxidase;Myeloperoxidase light chain;Myeloperoxidase heavy chain;MPO;
Subcellular Localization Lysosome.
Molecular Weight 83869 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Research Areas Cancer, Cancer Metabolism, Cardiovascular, Immunology, Innate Immunity, Macrophage / Inflamm., Metabolism, Pathways And Processes, Redox Metabolism, Vasculature

*You can search these to find other products in these research areas.
Background Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697(A49-S745) amino acids with the molecular mass of 80KDa.

Other Recommended Resources

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Order Product (PB9057)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

$50 fee for conjugation. Antibody size is reduced to 50ug.

Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships next business day.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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We promise all of our products perform as described in datasheets.

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Publications

The Gastric Mucosa from Patients Infected with CagA or VacA Helicobacter pylori Has a Lower Level of Dual Oxidase-2 Expression than Uninfected or %u2026
Lactobacillus acidophilus alleviates pouchitis after ileal pouch-anal anastomosis in rats
Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice
Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke
Wang W, Liu W, Liu J, Wang Z, Fan F, Ma Y, Jin C, Xiang Y, Huang Y, Zhang X, Xu W, Yin Y, He Y. Infect Immun. 2017 Sep 20;85(10). pii: e00438-17. doi: 10.1128/IAI.00438-17. Print 2017 Oct. Interleukin-17A Aggravates Middle Ear Injury Induced by St...
Wei CC, Kong YY, Li GQ, Guan YF, Wang P, Miao CY. Sci Rep. 2017 Apr 6;7(1):717. doi: 10.1038/s41598-017-00851-z. Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway.

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is mpo antibody