Product Info Summary
SKU: | M00372 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody
View all Myeloperoxidase/MPO Antibodies
SKU/Catalog Number
M00372
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody catalog # M00372. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00372)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOCF-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myeloperoxidase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00372 is reactive to MPO in Human
Applications
M00372 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
150 kDa
Calculated molecular weight
83.869kDa
Background of Myeloperoxidase/MPO
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Myeloperoxidase expression in HL-60 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MPO (Source: Uniprot.org, NCBI)
Gene Name
MPO
Full Name
Myeloperoxidase
Weight
83.869kDa
Superfamily
peroxidase family
Alternative Names
EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase MPO myeloperoxidase myeloperoxidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MPO, check out the MPO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody (M00372)
Hello CJ!
M00372 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Gastric Mucosa from Patients Infected with CagA or VacA Helicobacter pylori Has a Lower Level of Dual Oxidase-2 Expression than Uninfected or %u2026
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody
Question
My boss were well pleased with the WB result of your anti-Myeloperoxidase Rabbit Monoclonal antibody. However we have observed positive staining in liver lysosome. using this antibody. Is that expected? Could you tell me where is MPO supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
From literature, liver does express MPO. Generally MPO expresses in lysosome. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2020-02-12
Question
We are currently using anti-Myeloperoxidase Rabbit Monoclonal antibody M00372 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
The anti-Myeloperoxidase Rabbit Monoclonal antibody (M00372) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-02
Question
We have seen staining in human leukemia. Are there any suggestions? Is anti-Myeloperoxidase Rabbit Monoclonal antibody supposed to stain leukemia positively?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
From literature leukemia does express MPO. From Uniprot.org, MPO is expressed in bone marrow cell, leukemia, leukocyte, plasma, saliva, liver, among other tissues. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-06-20
Question
We bought anti-Myeloperoxidase Rabbit Monoclonal antibody for IF on leukocyte a few months ago. I am using human, and We want to use the antibody for WB next. My question regards examining leukocyte as well as bone marrow cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
B. Bhatt
Verified customer
Asked: 2016-09-15
Answer
I took a look at the website and datasheets of our anti-Myeloperoxidase Rabbit Monoclonal antibody and I see that M00372 has been validated on human in both IF and WB. Thus M00372 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-09-15