Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody

Myeloperoxidase/MPO antibody

Boster Bio Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody catalog # M00372. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00372
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody

View all Myeloperoxidase/MPO Antibodies

SKU/Catalog Number

M00372

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody catalog # M00372. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00372)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOCF-13

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Myeloperoxidase

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00372 is reactive to MPO in Human

Applications

M00372 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

150 kDa

Calculated molecular weight

83.869kDa

Background of Myeloperoxidase/MPO

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For MPO (Source: Uniprot.org, NCBI)

Gene Name

MPO

Full Name

Myeloperoxidase

Weight

83.869kDa

Superfamily

peroxidase family

Alternative Names

EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase MPO myeloperoxidase myeloperoxidase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MPO, check out the MPO Infographic

MPO infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00372 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The Gastric Mucosa from Patients Infected with CagA or VacA Helicobacter pylori Has a Lower Level of Dual Oxidase-2 Expression than Uninfected or %u2026

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4 Customer Q&As for Anti-Myeloperoxidase MPO Rabbit Monoclonal Antibody

Question

My boss were well pleased with the WB result of your anti-Myeloperoxidase Rabbit Monoclonal antibody. However we have observed positive staining in liver lysosome. using this antibody. Is that expected? Could you tell me where is MPO supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

From literature, liver does express MPO. Generally MPO expresses in lysosome. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002

Boster Scientific Support

Answered: 2020-02-12

Question

We are currently using anti-Myeloperoxidase Rabbit Monoclonal antibody M00372 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

The anti-Myeloperoxidase Rabbit Monoclonal antibody (M00372) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-02

Question

We have seen staining in human leukemia. Are there any suggestions? Is anti-Myeloperoxidase Rabbit Monoclonal antibody supposed to stain leukemia positively?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

From literature leukemia does express MPO. From Uniprot.org, MPO is expressed in bone marrow cell, leukemia, leukocyte, plasma, saliva, liver, among other tissues. Regarding which tissues have MPO expression, here are a few articles citing expression in various tissues:
Leukemia, Pubmed ID: 2903767
Leukocyte, Pubmed ID: 1334087, 8390465, 2884926
Liver, Pubmed ID: 19159218
Plasma, Pubmed ID: 16335952
Saliva, Pubmed ID: 16740002

Boster Scientific Support

Answered: 2019-06-20

Question

We bought anti-Myeloperoxidase Rabbit Monoclonal antibody for IF on leukocyte a few months ago. I am using human, and We want to use the antibody for WB next. My question regards examining leukocyte as well as bone marrow cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

B. Bhatt

Verified customer

Asked: 2016-09-15

Answer

I took a look at the website and datasheets of our anti-Myeloperoxidase Rabbit Monoclonal antibody and I see that M00372 has been validated on human in both IF and WB. Thus M00372 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-09-15

Order DetailsPrice
M00372

100μL

$370
M00372-30ul

30μL sample

$99
M00372-carrier-free

Carrier Free

$370

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